dc.contributor.author | Leder, Lena | |
dc.contributor.author | Kolehmainen, Marjukka | |
dc.contributor.author | Narverud, Ingunn | |
dc.contributor.author | Dahlman, Ingrid | |
dc.contributor.author | Myhrstad, Mari C W | |
dc.contributor.author | de Mello, Vanessa D | |
dc.contributor.author | Paananen, Jussi | |
dc.contributor.author | Carlberg, Carsten | |
dc.contributor.author | Schwab, Ursula | |
dc.contributor.author | Herzig, Karl-Heinz | |
dc.contributor.author | Cloetens, Lieselotte | |
dc.contributor.author | Storm, Matilda U | |
dc.contributor.author | Hukkanen, Janne | |
dc.contributor.author | Savolainen, Markku J | |
dc.contributor.author | Rosqvist, Fredrik | |
dc.contributor.author | Hermansen, Kjeld | |
dc.contributor.author | Dragsted, Lars O | |
dc.contributor.author | Gunnarsdottir, Ingibjörg | |
dc.contributor.author | Thorsdottir, Inga | |
dc.contributor.author | Risérus, Ulf | |
dc.contributor.author | Åkesson, Björn | |
dc.contributor.author | Thoresen, Magne | |
dc.contributor.author | Arner, Peter | |
dc.contributor.author | Poutanen, Kaisa S | |
dc.contributor.author | Uusitupa, Matti | |
dc.contributor.author | Holven, Kirsten B | |
dc.contributor.author | Ulven, Stine M | |
dc.date.accessioned | 2016-03-22T05:14:30Z | |
dc.date.available | 2016-03-22T05:14:30Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Genes & Nutrition. 2016 Mar 17;11(1):3 | |
dc.identifier.uri | http://hdl.handle.net/10852/49970 | |
dc.description.abstract | Background
Diet has a great impact on the risk of developing features of metabolic syndrome (MetS), type 2 diabetes mellitus (T2DM), and cardiovascular diseases (CVD). We evaluated whether a long-term healthy Nordic diet (ND) can modify the expression of inflammation and lipid metabolism-related genes in peripheral blood mononuclear cells (PBMCs) during a 2-h oral glucose tolerance test (OGTT) in individuals with MetS.
Methods
A Nordic multicenter randomized dietary study included subjects (n = 213) with MetS, randomized to a ND group or a control diet (CD) group applying an isocaloric study protocol. In this sub-study, we included subjects (n = 89) from three Nordic centers: Kuopio (n = 26), Lund (n = 30), and Oulu (n = 33) with a maximum weight change of ±4 kg, high-sensitivity C-reactive protein concentration ≤10 mg L−1, and baseline body mass index <39 kg m−2. PBMCs were isolated, and the mRNA gene expression analysis was measured by quantitative real-time polymerase chain reaction (qPCR). We analyzed the mRNA expression changes of 44 genes before and after a 2hOGTT at the beginning and the end of the intervention.
Results
The healthy ND significantly down-regulated the expression of toll-like receptor 4 (TLR4), interleukin 18 (IL18), and thrombospondin receptor (CD36) mRNA transcripts and significantly up-regulated the expression of peroxisome proliferator-activated receptor delta (PPARD) mRNA transcript after the 2hOGTT compared to the CD.
Conclusions
A healthy ND is able to modify the gene expression in PBMCs after a 2hOGTT. However, more studies are needed to clarify the biological and clinical relevance of these findings. | |
dc.language.iso | eng | |
dc.relation.ispartof | Lena Leder (2017) Healthy dietary patterns, lipids and inflammation in human randomized controlled trials. Doctoral thesis. http://urn.nb.no/URN:NBN:no-58152 | |
dc.relation.uri | http://urn.nb.no/URN:NBN:no-58152 | |
dc.rights | The Author(s); licensee BioMed Central Ltd. | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Effects of a healthy Nordic diet on gene expression changes in peripheral blood mononuclear cells in response to an oral glucose tolerance test in subjects with metabolic syndrome: a SYSDIET sub-study | |
dc.type | Journal article | |
dc.date.updated | 2016-03-22T05:14:31Z | |
dc.creator.author | Leder, Lena | |
dc.creator.author | Kolehmainen, Marjukka | |
dc.creator.author | Narverud, Ingunn | |
dc.creator.author | Dahlman, Ingrid | |
dc.creator.author | Myhrstad, Mari C W | |
dc.creator.author | de Mello, Vanessa D | |
dc.creator.author | Paananen, Jussi | |
dc.creator.author | Carlberg, Carsten | |
dc.creator.author | Schwab, Ursula | |
dc.creator.author | Herzig, Karl-Heinz | |
dc.creator.author | Cloetens, Lieselotte | |
dc.creator.author | Storm, Matilda U | |
dc.creator.author | Hukkanen, Janne | |
dc.creator.author | Savolainen, Markku J | |
dc.creator.author | Rosqvist, Fredrik | |
dc.creator.author | Hermansen, Kjeld | |
dc.creator.author | Dragsted, Lars O | |
dc.creator.author | Gunnarsdottir, Ingibjörg | |
dc.creator.author | Thorsdottir, Inga | |
dc.creator.author | Risérus, Ulf | |
dc.creator.author | Åkesson, Björn | |
dc.creator.author | Thoresen, Magne | |
dc.creator.author | Arner, Peter | |
dc.creator.author | Poutanen, Kaisa S | |
dc.creator.author | Uusitupa, Matti | |
dc.creator.author | Holven, Kirsten B | |
dc.creator.author | Ulven, Stine M | |
dc.identifier.doi | http://dx.doi.org/10.1186/s12263-016-0521-4 | |
dc.identifier.urn | URN:NBN:no-53673 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/49970/1/12263_2016_Article_521.pdf | |
dc.type.version | PublishedVersion | |
cristin.articleid | 3 | |