dc.contributor.author | Nordgård, Oddmund | |
dc.contributor.author | Andersen, Tor Ø | |
dc.contributor.author | Gabrielsen, Odd S | |
dc.date.accessioned | 2015-10-09T02:12:39Z | |
dc.date.available | 2015-10-09T02:12:39Z | |
dc.date.issued | 2004 | |
dc.identifier.citation | BMC Biochemistry. 2004 Nov 04;5(1):15 | |
dc.identifier.uri | http://hdl.handle.net/10852/46755 | |
dc.description.abstract | Background
The transcription factor c-Myb is expressed in hematopoietic progenitor cells and other rapidly proliferating tissues, regulating genes important for proliferation, differentiation and survival. The DNA-binding domain (DBD) of c-Myb contains three tandemly arranged imperfect repeats, designated Myb domain R1, R2 and R3. The three-dimensional structure of the DBD shows that only the second and third Myb domains are directly involved in sequence-specific DNA-binding, while the R1 repeat does not contact DNA and only marginally affects DNA-binding properties. No structural information is available on the N-terminal 30 residues. Since deletion of the N-terminal region including R1 plays an important role in oncogenic activation of c-Myb, we asked whether this region confers properties beyond DNA-binding to the neighbouring c-Myb DBD.
Results
Analysis of a putative RNA-binding function of c-Myb DBD revealed that poly(G) preferentially inhibited c-Myb DNA-binding. A strong sequence-selectivity was observed when different RNA polymers were compared. Most interesting, the poly(G) sensitivity was significantly larger for a protein containing the N-terminus and the R1-repeat than for the minimal DNA-binding domain.
Conclusion
Preferential inhibition of c-Myb DNA binding by poly(G) RNA suggests that c-Myb is able to interact with RNA in a sequence-selective manner. While R2 and R3, but not R1, are necessary for DNA-binding, R1 seems to have a distinct role in enhancing the RNA-sensitivity of c-Myb. | |
dc.language.iso | eng | |
dc.rights | Nordgård et al; licensee BioMed Central Ltd. | |
dc.rights | Attribution 2.0 Generic | |
dc.rights.uri | http://creativecommons.org/licenses/by/2.0/ | |
dc.title | Selective inhibition of c-Myb DNA-binding by RNA polymers | |
dc.type | Journal article | |
dc.date.updated | 2015-10-09T02:12:39Z | |
dc.creator.author | Nordgård, Oddmund | |
dc.creator.author | Andersen, Tor Ø | |
dc.creator.author | Gabrielsen, Odd S | |
dc.identifier.doi | http://dx.doi.org/10.1186/1471-2091-5-15 | |
dc.identifier.urn | URN:NBN:no-50936 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/46755/1/12858_2004_Article_85.pdf | |
dc.type.version | PublishedVersion | |
cristin.articleid | 15 | |