Fast genomic μChIP-chip from 1,000 cells

dc.contributor.author	Dahl, John A
dc.contributor.author	Reiner, Andrew H
dc.contributor.author	Collas, Philippe
dc.date.accessioned	2015-10-09T02:11:21Z
dc.date.available	2015-10-09T02:11:21Z
dc.date.issued	2009
dc.identifier.citation	Genome Biology. 2009 Feb 10;10(2):R13
dc.identifier.uri	http://hdl.handle.net/10852/46700
dc.description.abstract	Genome-wide location analysis of histone modifications and transcription factor binding relies on chromatin immunoprecipitation (ChIP) assays. These assays are, however, time-consuming and require large numbers of cells, hindering their application to the analysis of many interesting cell types. We report here a fast microChIP (μChIP) assay for 1,000 cells in combination with microarrays to produce genome-scale surveys of histone modifications. μChIP-chip reliably reproduces data obtained by large-scale assays: H3K9ac and H3K9m3 enrichment profiles are conserved and nucleosome-free regions are revealed.
dc.language.iso	eng
dc.rights	Dahl et al; licensee BioMed Central Ltd.
dc.rights	Attribution 2.0 Generic
dc.rights.uri	http://creativecommons.org/licenses/by/2.0/
dc.title	Fast genomic μChIP-chip from 1,000 cells
dc.type	Journal article
dc.date.updated	2015-10-09T02:11:21Z
dc.creator.author	Dahl, John A
dc.creator.author	Reiner, Andrew H
dc.creator.author	Collas, Philippe
dc.identifier.doi	http://dx.doi.org/10.1186/gb-2009-10-2-r13
dc.identifier.urn	URN:NBN:no-50870
dc.type.document	Tidsskriftartikkel
dc.type.peerreviewed	Peer reviewed
dc.identifier.fulltext	Fulltext https://www.duo.uio.no/bitstream/handle/10852/46700/1/13059_2008_Article_2163.pdf
dc.type.version	PublishedVersion
cristin.articleid	R13