dc.contributor.author | Ngai, Jacob | |
dc.contributor.author | Inngjerdingen, Marit | |
dc.contributor.author | Berge, Torunn | |
dc.contributor.author | Taskén, Kjetil | |
dc.date.accessioned | 2015-10-09T02:10:31Z | |
dc.date.available | 2015-10-09T02:10:31Z | |
dc.date.issued | 2009 | |
dc.identifier.citation | BMC Immunology. 2009 May 08;10(1):27 | |
dc.identifier.uri | http://hdl.handle.net/10852/46665 | |
dc.description.abstract | Background
TCR and CXCR4-mediated signaling appears to be reciprocally regulated pathways. TCR activation dampens the chemotactic response towards the CXCR4 ligand CXCL12, while T cells exposed to CXCL12 are less prone to subsequent TCR-activation. The heterotrimeric G proteins Gαq and Gαi2 have been implicated in CXCR4-signaling and we have recently also reported the possible involvement of Gαq in TCR-dependent activation of Lck (Ngai et al., Eur. J. Immunol., 2008, 38: 32083218). Here we examined the role of Gαq in migration and TCR activation.
Results
Pre-treatment of T cells with CXCL12 led to significantly reduced Lck Y394 phosphorylation upon TCR triggering indicating heterologous desensitization. We show that knockdown of Gαq significantly enhanced basal migration in T cells and reduced CXCL12-induced SHP-1 phosphorylation whereas Gαi2 knockdown inhibited CXCL12-induced migration.
Conclusion
Our data suggest that Gαi2 confers migration signals in the presence of CXCL12 whereas Gαq exerts a tonic inhibition on both basal and stimulated migrational responses. This is compatible with the notion that the level of Gαq activation contributes to determining the commitment of the T cell either to migration or activation through the TCR. | |
dc.language.iso | eng | |
dc.rights | Ngai et al. | |
dc.rights | Attribution 2.0 Generic | |
dc.rights.uri | http://creativecommons.org/licenses/by/2.0/ | |
dc.title | Interplay between the heterotrimeric G-protein subunits Gαq and Gαi2 sets the threshold for chemotaxis and TCR activation | |
dc.type | Journal article | |
dc.date.updated | 2015-10-09T02:10:31Z | |
dc.creator.author | Ngai, Jacob | |
dc.creator.author | Inngjerdingen, Marit | |
dc.creator.author | Berge, Torunn | |
dc.creator.author | Taskén, Kjetil | |
dc.identifier.doi | http://dx.doi.org/10.1186/1471-2172-10-27 | |
dc.identifier.urn | URN:NBN:no-50836 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/46665/1/12865_2008_Article_247.pdf | |
dc.type.version | PublishedVersion | |
cristin.articleid | 27 | |