dc.contributor.author | Mousavi, Seyed A | |
dc.contributor.author | Fønhus, Marita S | |
dc.contributor.author | Berg, Trond | |
dc.date.accessioned | 2015-10-09T01:58:33Z | |
dc.date.available | 2015-10-09T01:58:33Z | |
dc.date.issued | 2009 | |
dc.identifier.citation | BMC Cell Biology. 2009 May 11;10(1):39 | |
dc.identifier.uri | http://hdl.handle.net/10852/46549 | |
dc.description.abstract | Background
The urokinase plasminogen activator receptor associated protein (uPARAP)/Endo180 is a novel endocytic receptor that mediates collagen uptake and is implicated to play a role in physiological and pathological tissue-remodelling processes by mediating intracellular collagen degradation.
Result
This study investigates the expression of uPARAP/Endo180 protein and messenger RNA in primary rat hepatic stellate cell (HSC) cultures. The results show that uPARAP/Endo180 protein is not expressed in freshly isolated HSCs or during the first few days of culture while the cells still display quiescent features. In contrast, uPARAP/Endo180 protein is expressed early during HSC activation when cells are transdifferentiated into myofibroblast-like cells. Very low levels of uPARAP/Endo180 mRNA are detectable during the first days of culture but uPARAP/Endo180 mRNA is strongly up-regulated with increasing time in culture. Moreover, endocytic uptake of denatured collagen increases as transdifferentiation proceeds over time and correlates with increased expression of uPARAP/Endo180. Finally, analysis of uPARAP/Endo180 expression in four hepatic stellate cell lines from three different species showed that all these cell lines express uPARAP/Endo180 and are able to take up denatured collagen efficiently.
Conclusion
These results demonstrate that uPARAP/Endo180 expression by rat HSCs is strongly up-regulated during culture activation and identify this receptor as a feature common to culture-activated HSCs. | |
dc.language.iso | eng | |
dc.rights | Mousavi et al. | |
dc.rights | Attribution 2.0 Generic | |
dc.rights.uri | http://creativecommons.org/licenses/by/2.0/ | |
dc.title | Up-regulation of uPARAP/Endo180 during culture activation of rat hepatic stellate cells and its presence in hepatic stellate cell lines from different species | |
dc.type | Journal article | |
dc.date.updated | 2015-10-09T01:58:33Z | |
dc.creator.author | Mousavi, Seyed A | |
dc.creator.author | Fønhus, Marita S | |
dc.creator.author | Berg, Trond | |
dc.identifier.doi | http://dx.doi.org/10.1186/1471-2121-10-39 | |
dc.identifier.urn | URN:NBN:no-50735 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/46549/1/12860_2008_Article_388.pdf | |
dc.type.version | PublishedVersion | |
cristin.articleid | 39 | |