Abstract
The biotechnological transformation of underutilized resources, through processes like enzymatic hydrolysis, can contribute to the production of value-added products by using biomolecules (enzymes) to modify raw materials. Such value-added products can be, for example, nutraceuticals produced from food processing by-products. This dissertation demonstrated that enzymatic protein hydrolysis of chicken processing by-products can produce bioactive peptides with promising health-promoting effects. Bioanalytical methods were employed to screen, identify, and characterize bioactive peptides in a complex mixture of a crude protein hydrolysate. The bioactivities explored were blood pressure- and blood glucose regulation as well as antioxidant activity. Three bioactive peptides were identified from a mechanically deboned chicken residue (MDCR) hydrolysate. Our findings indicate that MDCR is a promising raw material to produce bioactive peptides. A low molecular weight peptide fraction isolated from the crude hydrolysate demonstrated a dual pharmacological effect (blood pressure- and blood glucose regulation). Dual activity is particularly valuable for management of complex diseases (e.g., diabetes) often requiring multi-drug regimen. Bioavailability of the identified bioactive peptides was evaluated. While the bioactive peptides demonstrated good gastrointestinal stability, their intestinal permeability could not be demonstrated. The dissertation demonstrated a promising potential of chicken by-product hydrolysates as a source of health-promoting bioactive peptides.
List of papers
Paper I: Liudmila Sorokina, Anne Rieder, Shiori Koga, Nils Kristian Afseth, Rita De Cássia Lemos Lima, Steven Ray Wilson and Sileshi Gizachew Wubshet. Multivariate correlation of infrared fingerprints and molecular weight distributions with bioactivity of poultry by-product protein hydrolysates. Journal of Functional Foods 95, 105170 (2022). DOI: 10.1016/j.jff.2022.105170. The article is included in the thesis. Also available at: https://doi.org/10.1016/j.jff.2022.105170 |
Paper II: Liudmila Sorokina, Josipa Matic, Anne Rieder, Shiori Koga, Nils Kristian Afseth, Steven Ray Wilson and Sileshi Gizachew Wubshet. Low Molecular Weight Peptide Fraction from Poultry Byproduct Hydrolysate Features Dual ACE-1 and DPP4 Inhibition. ACS Food Science & Technology 3, 2219-2228 (2023). DOI: 10.1021/acsfoodscitech.3c00417. The paper is not available in DUO due to publisher restrictions. The published version is available at: https://doi.org/10.1021/acsfoodscitech.3c00417 |
Paper III: Liudmila Sorokina, Anne Rieder, Nina Therese Solberg, Shiori Koga, Sissel Beate Rønning, Nils Kristian Afseth, Steven Ray Wilson and Sileshi Gizachew Wubshet. In vitro gastrointestinal stability and intestinal absorption of ACE-1 and DPP4 inhibitory peptides from poultry by-product hydrolysate. Prepared for submission. The paper is not available in DUO awaiting publishing |