dc.date.accessioned | 2023-02-10T18:27:42Z | |
dc.date.available | 2023-02-10T18:27:42Z | |
dc.date.created | 2022-04-29T12:53:20Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | An, Dongchen Pinheiro-Junior, Ernesto Lopes Béress, László Gladkikh, Irina Leychenko, Elena Undheim, Eivind Andreas Baste Peigneur, Steve Tytgat, Jan . AsKC11, a Kunitz Peptide from Anemonia sulcata, Is a Novel Activator of G Protein-Coupled Inward-Rectifier Potassium Channels. Marine Drugs. 2022, 20(2) | |
dc.identifier.uri | http://hdl.handle.net/10852/99889 | |
dc.description.abstract | (1) Background: G protein-coupled inward-rectifier potassium (GIRK) channels, especially neuronal GIRK1/2 channels, have been the focus of intense research interest for developing drugs against brain diseases. In this context, venom peptides that selectively activate GIRK channels can be seen as a new source for drug development. Here, we report on the identification and electrophysiological characterization of a novel activator of GIRK1/2 channels, AsKC11, found in the venom of the sea anemone Anemonia sulcata. (2) Methods: AsKC11 was purified from the sea anemone venom by reverse-phase chromatography and the sequence was identified by mass spectrometry. Using the two-electrode voltage-clamp technique, the activity of AsKC11 on GIRK1/2 channels was studied and its selectivity for other potassium channels was investigated. (3) Results: AsKC11, a Kunitz peptide found in the venom of A. sulcata, is the first peptide shown to directly activate neuronal GIRK1/2 channels independent from Gi/o protein activity, without affecting the inward-rectifier potassium channel (IRK1) and with only a minor effect on KV1.6 channels. Thus, AsKC11 is a novel activator of GIRK channels resulting in larger K+ currents because of an increased chord conductance. (4) Conclusions: These discoveries provide new insights into a novel class of GIRK activators. | |
dc.language | EN | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | AsKC11, a Kunitz Peptide from Anemonia sulcata, Is a Novel Activator of G Protein-Coupled Inward-Rectifier Potassium Channels | |
dc.title.alternative | ENEngelskEnglishAsKC11, a Kunitz Peptide from Anemonia sulcata, Is a Novel Activator of G Protein-Coupled Inward-Rectifier Potassium Channels | |
dc.type | Journal article | |
dc.creator.author | An, Dongchen | |
dc.creator.author | Pinheiro-Junior, Ernesto Lopes | |
dc.creator.author | Béress, László | |
dc.creator.author | Gladkikh, Irina | |
dc.creator.author | Leychenko, Elena | |
dc.creator.author | Undheim, Eivind Andreas Baste | |
dc.creator.author | Peigneur, Steve | |
dc.creator.author | Tytgat, Jan | |
cristin.unitcode | 185,15,29,50 | |
cristin.unitname | Centre for Ecological and Evolutionary Synthesis | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 2020126 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Marine Drugs&rft.volume=20&rft.spage=&rft.date=2022 | |
dc.identifier.jtitle | Marine Drugs | |
dc.identifier.volume | 20 | |
dc.identifier.issue | 2 | |
dc.identifier.pagecount | 15 | |
dc.identifier.doi | https://doi.org/10.3390/md20020140 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 1660-3397 | |
dc.type.version | PublishedVersion | |
cristin.articleid | 140 | |