Titers of antibodies against ancestral SARS-CoV-2 correlate with levels of neutralizing antibodies to multiple variants
dc.date.accessioned | 2023-01-09T18:26:47Z | |
dc.date.available | 2023-01-09T18:26:47Z | |
dc.date.created | 2023-01-04T16:05:38Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | Tran, Trung The Vaage, Eline Benno Mehta, Adi Chopra, Adity Tietze, Lisa Kolderup, Anette Anthi, Aina Karen König, Marton Nygaard, Gro Owren Lind, Andreas Müller, Fredrik Nissen-Meyer, Lise Sofie Haug Magnus, Per Minor Trogstad, Lill Mjaaland, Siri Søraas, Arne Vasli Midtvedt, Karsten Åsberg, Anders Barratt-Due, Andreas Medhus, Asle Wilhelm Høivik, Marte Lie Lundin, Knut Karlsen, Randi Fuglaas Dahle, Reidun Danielsson, Karin Thomassen, Kristine Stien Kro, Grete Anette Birkeland Cox, Rebecca Jane Zhou, Fan Langeland, Nina Aukrust, Pål Melum, Espen Åvitsland, Tone Lise Wiencke, Kristine Holter, Jan Cato Munthe, Ludvig Andre Grødeland, Gunnveig Andersen, Jan Terje Vaage, John T. Lund-Johansen, Fridtjof . Titers of antibodies against ancestral SARS-CoV-2 correlate with levels of neutralizing antibodies to multiple variants. npj Vaccines. 2022, 7(1) | |
dc.identifier.uri | http://hdl.handle.net/10852/98610 | |
dc.description.abstract | Abstract Diagnostic assays currently used to monitor the efficacy of COVID-19 vaccines measure levels of antibodies to the receptor-binding domain of ancestral SARS-CoV-2 (RBDwt). However, the predictive value for protection against new variants of concern (VOCs) has not been firmly established. Here, we used bead-based arrays and flow cytometry to measure binding of antibodies to spike proteins and receptor-binding domains (RBDs) from VOCs in 12,000 serum samples. Effects of sera on RBD-ACE2 interactions were measured as a proxy for neutralizing antibodies. The samples were obtained from healthy individuals or patients on immunosuppressive therapy who had received two to four doses of COVID-19 vaccines and from COVID-19 convalescents. The results show that anti-RBDwt titers correlate with the levels of binding- and neutralizing antibodies against the Alpha, Beta, Gamma, Delta, Epsilon and Omicron variants. The benefit of multiplexed analysis lies in the ability to measure a wide range of anti-RBD titers using a single dilution of serum for each assay. The reactivity patterns also yield an internal reference for neutralizing activity and binding antibody units per milliliter (BAU/ml). Results obtained with sera from vaccinated healthy individuals and patients confirmed and extended results from previous studies on time-dependent waning of antibody levels and effects of immunosuppressive agents. We conclude that anti-RBDwt titers correlate with levels of neutralizing antibodies against VOCs and propose that our method may be implemented to enhance the precision and throughput of immunomonitoring. | |
dc.language | EN | |
dc.publisher | Nature Portfolio | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Titers of antibodies against ancestral SARS-CoV-2 correlate with levels of neutralizing antibodies to multiple variants | |
dc.title.alternative | ENEngelskEnglishTiters of antibodies against ancestral SARS-CoV-2 correlate with levels of neutralizing antibodies to multiple variants | |
dc.type | Journal article | |
dc.creator.author | Tran, Trung The | |
dc.creator.author | Vaage, Eline Benno | |
dc.creator.author | Mehta, Adi | |
dc.creator.author | Chopra, Adity | |
dc.creator.author | Tietze, Lisa | |
dc.creator.author | Kolderup, Anette | |
dc.creator.author | Anthi, Aina Karen | |
dc.creator.author | König, Marton | |
dc.creator.author | Nygaard, Gro Owren | |
dc.creator.author | Lind, Andreas | |
dc.creator.author | Müller, Fredrik | |
dc.creator.author | Nissen-Meyer, Lise Sofie Haug | |
dc.creator.author | Magnus, Per Minor | |
dc.creator.author | Trogstad, Lill | |
dc.creator.author | Mjaaland, Siri | |
dc.creator.author | Søraas, Arne Vasli | |
dc.creator.author | Midtvedt, Karsten | |
dc.creator.author | Åsberg, Anders | |
dc.creator.author | Barratt-Due, Andreas | |
dc.creator.author | Medhus, Asle Wilhelm | |
dc.creator.author | Høivik, Marte Lie | |
dc.creator.author | Lundin, Knut | |
dc.creator.author | Karlsen, Randi Fuglaas | |
dc.creator.author | Dahle, Reidun | |
dc.creator.author | Danielsson, Karin | |
dc.creator.author | Thomassen, Kristine Stien | |
dc.creator.author | Kro, Grete Anette Birkeland | |
dc.creator.author | Cox, Rebecca Jane | |
dc.creator.author | Zhou, Fan | |
dc.creator.author | Langeland, Nina | |
dc.creator.author | Aukrust, Pål | |
dc.creator.author | Melum, Espen | |
dc.creator.author | Åvitsland, Tone Lise | |
dc.creator.author | Wiencke, Kristine | |
dc.creator.author | Holter, Jan Cato | |
dc.creator.author | Munthe, Ludvig Andre | |
dc.creator.author | Grødeland, Gunnveig | |
dc.creator.author | Andersen, Jan Terje | |
dc.creator.author | Vaage, John T. | |
dc.creator.author | Lund-Johansen, Fridtjof | |
cristin.unitcode | 185,53,18,12 | |
cristin.unitname | Immunologi og transfusjonsmedisin | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 2100792 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=npj Vaccines&rft.volume=7&rft.spage=&rft.date=2022 | |
dc.identifier.jtitle | npj Vaccines | |
dc.identifier.volume | 7 | |
dc.identifier.issue | 1 | |
dc.identifier.pagecount | 11 | |
dc.identifier.doi | https://doi.org/10.1038/s41541-022-00586-7 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 2059-0105 | |
dc.type.version | PublishedVersion | |
cristin.articleid | 174 |
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Farmasøytisk institutt [1966]
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Institutt for klinisk medisin [10950]
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