dc.date.accessioned | 2022-12-22T17:41:09Z | |
dc.date.available | 2022-12-22T17:41:09Z | |
dc.date.created | 2022-05-06T13:38:42Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | Su, Zhangli Monshaugen, Ida Wilson, Briana Wang, Fengbin Klungland, Arne Ougland, Rune Dutta, Anindya . TRMT6/61A-dependent base methylation of tRNA-derived fragments regulates gene-silencing activity and the unfolded protein response in bladder cancer. Nature Communications. 2022, 13(1) | |
dc.identifier.uri | http://hdl.handle.net/10852/98325 | |
dc.description.abstract | Abstract RNA modifications are important regulatory elements of RNA functions. However, most genome-wide mapping of RNA modifications has focused on messenger RNAs and transfer RNAs, but such datasets have been lacking for small RNAs. Here we mapped N 1 -methyladenosine (m 1 A) in the cellular small RNA space. Benchmarked with synthetic m 1 A RNAs, our workflow identified specific groups of m 1 A-containing small RNAs, which are otherwise disproportionally under-represented. In particular, 22-nucleotides long 3′ tRNA-fragments are highly enriched for TRMT6/61A-dependent m 1 A located within the seed region. TRMT6/61A-dependent m 1 A negatively affects gene silencing by tRF-3s. In urothelial carcinoma of the bladder, where TRMT6/61A is over-expressed, higher m 1 A modification on tRFs is detected, correlated with a dysregulation of tRF targetome. Lastly, TRMT6/61A regulates tRF-3 targets involved in unfolded protein response. Together, our results reveal a mechanism of regulating gene expression via base modification of small RNA. | |
dc.language | EN | |
dc.publisher | Nature Portfolio | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | TRMT6/61A-dependent base methylation of tRNA-derived fragments regulates gene-silencing activity and the unfolded protein response in bladder cancer | |
dc.title.alternative | ENEngelskEnglishTRMT6/61A-dependent base methylation of tRNA-derived fragments regulates gene-silencing activity and the unfolded protein response in bladder cancer | |
dc.type | Journal article | |
dc.creator.author | Su, Zhangli | |
dc.creator.author | Monshaugen, Ida | |
dc.creator.author | Wilson, Briana | |
dc.creator.author | Wang, Fengbin | |
dc.creator.author | Klungland, Arne | |
dc.creator.author | Ougland, Rune | |
dc.creator.author | Dutta, Anindya | |
cristin.unitcode | 185,51,12,0 | |
cristin.unitname | Avdeling for molekylærmedisin | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 2 | |
dc.identifier.cristin | 2022132 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Nature Communications&rft.volume=13&rft.spage=&rft.date=2022 | |
dc.identifier.jtitle | Nature Communications | |
dc.identifier.volume | 13 | |
dc.identifier.issue | 1 | |
dc.identifier.pagecount | 0 | |
dc.identifier.doi | https://doi.org/10.1038/s41467-022-29790-8 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 2041-1723 | |
dc.type.version | PublishedVersion | |
cristin.articleid | 2165 | |