dc.date.accessioned | 2022-08-04T16:20:07Z | |
dc.date.available | 2022-08-04T16:20:07Z | |
dc.date.created | 2022-07-06T05:37:15Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | Vaca, Diana Thibau, Arno Leisegang, Matthias Malmström, Johan Linke, Dirk Eble, Johannes A. Ballhorn, Wibke Schaller, Martin Happonen, Lotta Johanna Kempf, Volkhard A. J. . Interaction of Bartonella henselae with Fibronectin Represents the Molecular Basis for Adhesion to Host Cells. Microbiology spectrum. 2022, 10(3) | |
dc.identifier.uri | http://hdl.handle.net/10852/94743 | |
dc.description.abstract | Bacterial adhesion to the host is the most decisive step in infections. Trimeric autotransporter adhesins (TAA) are important pathogenicity factors of Gram-negative bacteria. The prototypic TAA Bartonella adhesin A (BadA) from human-pathogenic Bartonella henselae mediates bacterial adherence to endothelial cells (ECs) and extracellular matrix proteins. Here, we determined the interaction between BadA and fibronectin (Fn) to be essential for bacterial host cell adhesion. BadA interactions occur within the heparin-binding domains of Fn. The exact binding sites were revealed by mass spectrometry analysis of chemically cross-linked whole-cell bacteria and Fn. Specific BadA interactions with defined Fn regions represent the molecular basis for bacterial adhesion to ECs and these data were confirmed by BadA-deficient bacteria and CRISPR-Cas knockout Fn host cells. Interactions between TAAs and the extracellular matrix might represent the key step for adherence of human-pathogenic Gram-negative bacteria to the host. | |
dc.language | EN | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Interaction of Bartonella henselae with Fibronectin Represents the Molecular Basis for Adhesion to Host Cells | |
dc.title.alternative | ENEngelskEnglishInteraction of Bartonella henselae with Fibronectin Represents the Molecular Basis for Adhesion to Host Cells | |
dc.type | Journal article | |
dc.creator.author | Vaca, Diana | |
dc.creator.author | Thibau, Arno | |
dc.creator.author | Leisegang, Matthias | |
dc.creator.author | Malmström, Johan | |
dc.creator.author | Linke, Dirk | |
dc.creator.author | Eble, Johannes A. | |
dc.creator.author | Ballhorn, Wibke | |
dc.creator.author | Schaller, Martin | |
dc.creator.author | Happonen, Lotta Johanna | |
dc.creator.author | Kempf, Volkhard A. J. | |
cristin.unitcode | 185,15,29,0 | |
cristin.unitname | Institutt for biovitenskap | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 2037311 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Microbiology spectrum&rft.volume=10&rft.spage=&rft.date=2022 | |
dc.identifier.jtitle | Microbiology spectrum | |
dc.identifier.volume | 10 | |
dc.identifier.issue | 3 | |
dc.identifier.doi | https://doi.org/10.1128/spectrum.00598-22 | |
dc.identifier.urn | URN:NBN:no-97288 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 2165-0497 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/94743/1/2022-Vaca-MicrobiolSpectrum%2BBadA%2BFibrinectin%2Bmass%2Bspec.pdf | |
dc.type.version | PublishedVersion | |
cristin.articleid | e00598-22 | |
dc.relation.project | EC/H2020/765042 | |