dc.date.accessioned | 2022-06-29T16:53:50Z | |
dc.date.available | 2022-06-29T16:53:50Z | |
dc.date.created | 2022-06-22T13:31:45Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | Schröder, Maria Elisabeth Reseland, Janne Elin Haugen, Håvard Jostein . Osteoblasts in a Perfusion Flow Bioreactor—Tissue Engineered Constructs of TiO2 Scaffolds and Cells for Improved Clinical Performance. Cells. 2022, 11(13), 1995 | |
dc.identifier.uri | http://hdl.handle.net/10852/94512 | |
dc.description.abstract | Combining biomaterial scaffolds with cells serves as a promising strategy for engineering critical size defects; however, homogenous cellular growth within large scaffolds is challenging. Mechanical stimuli can enhance bone regeneration by modulating cellular growth and differentiation. Here, we compare dynamic seeding in a perfusion flow bioreactor with static seeding for a synthetic bone scaffold for up to 21 days using the cell line MC3T3-E1 and primary human osteoblast, confocal laser scanning microscopy, and real-time reverse transcriptase-polymerase chain reaction. The secretion of bone-related proteins was quantified using multiplex immunoassays. Dynamic culture improved cellular distribution through the TiO2 scaffold and induced a five-fold increase in cell number after 21 days. The relative mRNA expression of osteopontin of MC3T3-E1 was 40-fold enhanced after 7 and 21 days at a flow rate of 0.08 mL/min, and that of collagen type I alpha I expression was 18-fold after 21 days. A flow rate of 0.16 mL/min was 10-fold less effective. Dynamic culture increased the levels of dickkopf-related protein 1 (60-fold), osteoprotegrin (29-fold), interleukin-6 (23-fold), interleukin-8 (36-fold), monocyte chemoattractant protein 1 (28-fold) and vascular endothelial growth factor (6-fold) in the medium of primary human osteoblasts after 21 days compared to static seeding. The proposed method may have clinical potential for bone tissue engineering. | |
dc.language | EN | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Osteoblasts in a Perfusion Flow Bioreactor—Tissue Engineered Constructs of TiO2 Scaffolds and Cells for Improved Clinical Performance | |
dc.title.alternative | ENEngelskEnglishOsteoblasts in a Perfusion Flow Bioreactor—Tissue Engineered Constructs of TiO2 Scaffolds and Cells for Improved Clinical Performance | |
dc.type | Journal article | |
dc.creator.author | Schröder, Maria Elisabeth | |
dc.creator.author | Reseland, Janne Elin | |
dc.creator.author | Haugen, Håvard Jostein | |
cristin.unitcode | 185,16,17,62 | |
cristin.unitname | Biomaterialer | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 2034257 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Cells&rft.volume=11&rft.spage=1995&rft.date=2022 | |
dc.identifier.jtitle | Cells | |
dc.identifier.volume | 11 | |
dc.identifier.issue | 13 | |
dc.identifier.doi | https://doi.org/10.3390/cells11131995 | |
dc.identifier.urn | URN:NBN:no-97056 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 2073-4409 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/94512/1/cells-11-01995.pdf | |
dc.type.version | PublishedVersion | |
cristin.articleid | 1995 | |
dc.relation.project | NFR/287991 | |