dc.date.accessioned | 2022-03-02T17:45:50Z | |
dc.date.available | 2022-03-02T17:45:50Z | |
dc.date.created | 2021-11-17T10:21:47Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Wang, Katherine Esbensen, Qin Ying Karlsen, Tommy Aleksander Eftang, Cathrine Nørstad Owesen, Christian Årøen, Asbjørn Jakobsen, Rune Bruhn . Low-Input RNA-Sequencing in Patients with Cartilage Lesions, Osteoarthritis, and Healthy Cartilage. Cartilage. 2021, 13, 550S-562S | |
dc.identifier.uri | http://hdl.handle.net/10852/91698 | |
dc.description.abstract | Objective: To analyze and compare cartilage samples from 3 groups of patients utilizing low-input RNA-sequencing. Design: Cartilage biopsies were collected from patients in 3 groups (n = 48): Cartilage lesion (CL) patients had at least ICRS grade 2, osteoarthritis (OA) samples were taken from patients undergoing knee replacement, and healthy cartilage (HC) was taken from ACL-reconstruction patients without CLs. RNA was isolated using an optimized protocol. RNA samples were assessed for quality and sequenced with a low-input SmartSeq2 protocol. Results: RNA isolation yielded 48 samples with sufficient quality for sequencing. After quality control, 13 samples in the OA group, 9 in the HC group, and 9 in the CL group were included in the analysis. There was a high degree of co-clustering between the HC and CL groups with only 6 genes significantly up- or downregulated. OA and the combined HC/CL group clustered significantly separate from each other, yielding 659 significantly upregulated and 1,369 downregulated genes. GO-term analysis revealed that genes matched to cartilage and connective tissue development terms. Conclusion: The gene expression profiles from the 3 groups suggest that there are no major differences in gene expression between cartilage from knees with a cartilage injury and knees without an apparent cartilage injury. OA cartilage, as expected, showed markedly different gene expression from the other 2 groups. The gene expression profiles resulting from this low-input RNA-sequencing study offer opportunities to discover new pathways not previously recognized that may be explored in future studies. | |
dc.language | EN | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Low-Input RNA-Sequencing in Patients with Cartilage Lesions, Osteoarthritis, and Healthy Cartilage | |
dc.type | Journal article | |
dc.creator.author | Wang, Katherine | |
dc.creator.author | Esbensen, Qin Ying | |
dc.creator.author | Karlsen, Tommy Aleksander | |
dc.creator.author | Eftang, Cathrine Nørstad | |
dc.creator.author | Owesen, Christian | |
dc.creator.author | Årøen, Asbjørn | |
dc.creator.author | Jakobsen, Rune Bruhn | |
cristin.unitcode | 185,53,83,0 | |
cristin.unitname | Klinikk for kirurgiske fag | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 1955451 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Cartilage&rft.volume=13&rft.spage=550S&rft.date=2021 | |
dc.identifier.jtitle | Cartilage | |
dc.identifier.volume | 13 | |
dc.identifier.issue | 1_suppl | |
dc.identifier.startpage | 550S | |
dc.identifier.endpage | 562S | |
dc.identifier.doi | https://doi.org/10.1177/19476035211057245 | |
dc.identifier.urn | URN:NBN:no-94314 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 1947-6035 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/91698/1/19476035211057245.pdf | |
dc.type.version | PublishedVersion | |