The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders
dc.date.accessioned | 2022-02-25T18:38:38Z | |
dc.date.available | 2022-02-25T18:38:38Z | |
dc.date.created | 2021-11-30T09:48:31Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Milić, Mirta Ceppi, Marcello Bruzzone, Marco Azqueta, Amaya Brunborg, Gunnar Godschalk, Roger Koppen, Gudrun Langie, Sabine Møller, Peter Teixeira, João Paulo Alija, Avdulla Anderson, Diana Andrade, Vanessa Andreoli, Cristina Asllani, Fisnik Eyluel Bangkoglu, Ezgi Barancokova, Magdalena Basaran, Nursen Boutet-Robinet, Elisa Buschini, Annamaria Cavallo, Delia Costa Pererira, Cristina Costa, Carla Costa, Solange Da Silva, Juliana Del Bo’, Cristian Dimitrijevic Sreckovic, Vesna Djelic, Ninoslav Dobrzynska, Malgorzata Duracková, Zdenka Dvorakova, Monika Gajski, Goran Galati, Serena García Lima, Omar Giovannelli, Lisa Goroshinskaya, Irina A. Grindel, Annemarie Gützkow, Kristine Bjerve Hernández, Alba Hernandez, Carlos Holven, Kirsten Bjørklund Ibero-Baraibar, Idoia Ottestad, Inger Olaug Kadioglu, Ela Kazimirova, Alena Kuznetsova, Elena Ladeira, Carina Laffon, Blanca Lamonaca, Palma Lebailly, Pierre Louro, Henriqueta Cardoso, Tania Mandina Marcon, Francesca Marcos, Ricard Moretti, Massimo Moretti, Silvia Najafzadeh, Mojgan Nemeth, Zsuzsanna Neri, Monica Novotna, Bozena Orlow, Irene Paduchova, Zuzana Pastor, Susana Perdry, Hervé Spremo-Potparević, Biljana Ramadhani, Dwi Riso, Patrizia Rohr, Paula Rojas, Emilio Rossner, Pavel Safar, Anna Sardas, Semra Silva, Maria João Sirota, Nikolay Smolkova, Bozena Staruchova, Marta Stetina, Rudolf Stopper, Helga Surikova, Ekaterina I. Ulven, Stine Marie Ursini, Cinzia Lucia Valdiglesias, Vanessa Valverde, Mahara Vodička, Pavel Volkovova, Katarina Wagner, Karl-Heinz Zivkovic, Lada Dusinska, Maria Collins, Andrew Richard Bonassi, Stefano . The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders. Mutation research. Reviews in mutation research. 2021, 787 | |
dc.identifier.uri | http://hdl.handle.net/10852/91538 | |
dc.description.abstract | The alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project - a COST Action launched in 2016 - represent a valuable tool to meet this challenge. The aims of hCOMET were to establish reference values for the level of DNA damage in humans, to investigate the effect of host factors, lifestyle and exposure to genotoxic agents, and to compare different sources of assay variability. A database of 19,320 subjects was generated, pooling data from 105 studies run by 44 laboratories in 26 countries between 1999 and 2019. A mixed random effect log-linear model, in parallel with a classic meta-analysis, was applied to take into account the extensive heterogeneity of data, due to descriptor, specimen and protocol variability. As a result of this analysis interquartile intervals of DNA strand breaks (which includes alkali-labile sites) were reported for tail intensity, tail length, and tail moment (comet assay descriptors). A small variation by age was reported in some datasets, suggesting higher DNA damage in oldest age-classes, while no effect could be shown for sex or smoking habit, although the lack of data on heavy smokers has still to be considered. Finally, highly significant differences in DNA damage were found for most exposures investigated in specific studies. In conclusion, these data, which confirm that DNA damage measured by the comet assay is an excellent biomarker of exposure in several conditions, may contribute to improving the quality of study design and to the standardization of results of the comet assay in human populations. | |
dc.language | EN | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.title | The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders | |
dc.type | Journal article | |
dc.creator.author | Milić, Mirta | |
dc.creator.author | Ceppi, Marcello | |
dc.creator.author | Bruzzone, Marco | |
dc.creator.author | Azqueta, Amaya | |
dc.creator.author | Brunborg, Gunnar | |
dc.creator.author | Godschalk, Roger | |
dc.creator.author | Koppen, Gudrun | |
dc.creator.author | Langie, Sabine | |
dc.creator.author | Møller, Peter | |
dc.creator.author | Teixeira, João Paulo | |
dc.creator.author | Alija, Avdulla | |
dc.creator.author | Anderson, Diana | |
dc.creator.author | Andrade, Vanessa | |
dc.creator.author | Andreoli, Cristina | |
dc.creator.author | Asllani, Fisnik | |
dc.creator.author | Eyluel Bangkoglu, Ezgi | |
dc.creator.author | Barancokova, Magdalena | |
dc.creator.author | Basaran, Nursen | |
dc.creator.author | Boutet-Robinet, Elisa | |
dc.creator.author | Buschini, Annamaria | |
dc.creator.author | Cavallo, Delia | |
dc.creator.author | Costa Pererira, Cristina | |
dc.creator.author | Costa, Carla | |
dc.creator.author | Costa, Solange | |
dc.creator.author | Da Silva, Juliana | |
dc.creator.author | Del Bo’, Cristian | |
dc.creator.author | Dimitrijevic Sreckovic, Vesna | |
dc.creator.author | Djelic, Ninoslav | |
dc.creator.author | Dobrzynska, Malgorzata | |
dc.creator.author | Duracková, Zdenka | |
dc.creator.author | Dvorakova, Monika | |
dc.creator.author | Gajski, Goran | |
dc.creator.author | Galati, Serena | |
dc.creator.author | García Lima, Omar | |
dc.creator.author | Giovannelli, Lisa | |
dc.creator.author | Goroshinskaya, Irina A. | |
dc.creator.author | Grindel, Annemarie | |
dc.creator.author | Gützkow, Kristine Bjerve | |
dc.creator.author | Hernández, Alba | |
dc.creator.author | Hernandez, Carlos | |
dc.creator.author | Holven, Kirsten Bjørklund | |
dc.creator.author | Ibero-Baraibar, Idoia | |
dc.creator.author | Ottestad, Inger Olaug | |
dc.creator.author | Kadioglu, Ela | |
dc.creator.author | Kazimirova, Alena | |
dc.creator.author | Kuznetsova, Elena | |
dc.creator.author | Ladeira, Carina | |
dc.creator.author | Laffon, Blanca | |
dc.creator.author | Lamonaca, Palma | |
dc.creator.author | Lebailly, Pierre | |
dc.creator.author | Louro, Henriqueta | |
dc.creator.author | Cardoso, Tania Mandina | |
dc.creator.author | Marcon, Francesca | |
dc.creator.author | Marcos, Ricard | |
dc.creator.author | Moretti, Massimo | |
dc.creator.author | Moretti, Silvia | |
dc.creator.author | Najafzadeh, Mojgan | |
dc.creator.author | Nemeth, Zsuzsanna | |
dc.creator.author | Neri, Monica | |
dc.creator.author | Novotna, Bozena | |
dc.creator.author | Orlow, Irene | |
dc.creator.author | Paduchova, Zuzana | |
dc.creator.author | Pastor, Susana | |
dc.creator.author | Perdry, Hervé | |
dc.creator.author | Spremo-Potparević, Biljana | |
dc.creator.author | Ramadhani, Dwi | |
dc.creator.author | Riso, Patrizia | |
dc.creator.author | Rohr, Paula | |
dc.creator.author | Rojas, Emilio | |
dc.creator.author | Rossner, Pavel | |
dc.creator.author | Safar, Anna | |
dc.creator.author | Sardas, Semra | |
dc.creator.author | Silva, Maria João | |
dc.creator.author | Sirota, Nikolay | |
dc.creator.author | Smolkova, Bozena | |
dc.creator.author | Staruchova, Marta | |
dc.creator.author | Stetina, Rudolf | |
dc.creator.author | Stopper, Helga | |
dc.creator.author | Surikova, Ekaterina I. | |
dc.creator.author | Ulven, Stine Marie | |
dc.creator.author | Ursini, Cinzia Lucia | |
dc.creator.author | Valdiglesias, Vanessa | |
dc.creator.author | Valverde, Mahara | |
dc.creator.author | Vodička, Pavel | |
dc.creator.author | Volkovova, Katarina | |
dc.creator.author | Wagner, Karl-Heinz | |
dc.creator.author | Zivkovic, Lada | |
dc.creator.author | Dusinska, Maria | |
dc.creator.author | Collins, Andrew Richard | |
dc.creator.author | Bonassi, Stefano | |
cristin.unitcode | 185,51,13,20 | |
cristin.unitname | Seksjon for klinisk ernæring | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 1961405 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Mutation research. Reviews in mutation research&rft.volume=787&rft.spage=&rft.date=2021 | |
dc.identifier.jtitle | Mutation research. Reviews in mutation research | |
dc.identifier.volume | 787 | |
dc.identifier.pagecount | 13 | |
dc.identifier.doi | https://doi.org/10.1016/j.mrrev.2021.108371 | |
dc.identifier.urn | URN:NBN:no-94126 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 1383-5742 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/91538/1/1-s2.0-S1383574221000089-main.pdf | |
dc.type.version | PublishedVersion | |
cristin.articleid | 108371 |
Files in this item
Appears in the following Collection
-
Institutt for medisinske basalfag [2799]
-
CRIStin høstingsarkiv [31446]