dc.date.accessioned | 2022-02-12T19:15:46Z | |
dc.date.available | 2022-02-12T19:15:46Z | |
dc.date.created | 2022-01-20T13:32:17Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Wilson, Steven Ray Haakon Krauss, Stefan Johannes Karl Lundanes, Elsa Skottvoll, Frøydis Sved Bogen, Inger Lise Pedersen-Bjergaard, Stig Hansen, Frederik Aizenshtadt, Aleksandra Kutter, Jörg P. Andersen, Jannike Mørch Martinez, Mikel Amirola . Direct Electromembrane Extraction based Mass Spectrometry: A Tool for Studying Drug Metabolism Properties of Liver Organoids. Analysis & Sensing. 2021, 2(1), 1-5 | |
dc.identifier.uri | http://hdl.handle.net/10852/90886 | |
dc.description.abstract | This work introduces a strategy for organoid analysis - direct Electromembrane Extraction based Mass Spectrometry (dEME-MS) – for coupling liver organoids with mass spectrometry (MS). dEME-MS comprises electrophoresis of selected small molecules from a culture chamber across an oil membrane, and to a MS compatible solution. This enables clean micro-extraction of drugs and their metabolites as produced in the liver organoids to capillary liquid chromatography-mass spectrometry. Applying dEME-MS, proof-of-concept of directly measuring methadone metabolism is demonstrated on adult liver organoids. With 50 liver organoids and 1 μM methadone, methadone metabolism was monitored from 0 to 24 hours (11 time points). All analytes had <0.4 % variance in retention times with >100 measurements. dEME-MS is capable of automated and selective monitoring of drug metabolism in liver organoids, and could serve as a valuable tool for automated drug discovery efforts. | |
dc.language | EN | |
dc.rights | Attribution-NonCommercial 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc/4.0/ | |
dc.title | Direct Electromembrane Extraction based Mass Spectrometry: A Tool for Studying Drug Metabolism Properties of Liver Organoids | |
dc.type | Journal article | |
dc.creator.author | Wilson, Steven Ray Haakon | |
dc.creator.author | Krauss, Stefan Johannes Karl | |
dc.creator.author | Lundanes, Elsa | |
dc.creator.author | Skottvoll, Frøydis Sved | |
dc.creator.author | Bogen, Inger Lise | |
dc.creator.author | Pedersen-Bjergaard, Stig | |
dc.creator.author | Hansen, Frederik | |
dc.creator.author | Aizenshtadt, Aleksandra | |
dc.creator.author | Kutter, Jörg P. | |
dc.creator.author | Andersen, Jannike Mørch | |
dc.creator.author | Martinez, Mikel Amirola | |
cristin.unitcode | 185,15,12,63 | |
cristin.unitname | Seksjon for kjemisk livsvitenskap - biomolekyler, bio-inspirerte materialer og bioanalytisk kjemi | |
cristin.ispublished | true | |
cristin.fulltext | original | |
dc.identifier.cristin | 1986218 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Analysis & Sensing&rft.volume=2&rft.spage=1&rft.date=2021 | |
dc.identifier.jtitle | Analysis & Sensing | |
dc.identifier.volume | 2 | |
dc.identifier.issue | 1 | |
dc.identifier.startpage | 1 | |
dc.identifier.endpage | 5 | |
dc.identifier.doi | https://doi.org/10.1002/anse.202100051 | |
dc.identifier.urn | URN:NBN:no-93450 | |
dc.type.document | Tidsskriftartikkel | |
dc.source.issn | 2629-2742 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/90886/1/Analysis%2BSensing%2B-%2B2021%2B-%2BSkottvoll%2B-%2BDirect%2BElectromembrane%2BExtraction%2525u2010Based%2BMass%2BSpectrometry%2BA%2BTool%2Bfor%2BStudying%2BDrug.pdf | |
dc.type.version | PublishedVersion | |
dc.relation.project | NFR/262613 | |