dc.date.accessioned | 2022-02-02T18:22:40Z | |
dc.date.available | 2022-02-02T18:22:40Z | |
dc.date.created | 2022-01-06T11:38:32Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Reint, Ganna Li, Zhuokun Labun, Kornel Keskitalo, Salla Soppa, Inkeri Mamia, Katariina Aino Inkeri Tolo, Eero Szymanska, Monika Meza-Zepeda, Leonardo A. Lorenz, Susanne Cieslar-Pobuda, Artur Grzegorz Hu, Xian Bodin, Diana L Staerk, Judith Valen, Eivind Dale Schmierer, Bernhard Varjosalo, Markku Taipale, Jussi Haapaniemi, Emma Maria . Rapid genome editing by CRISPR-Cas9-POLD3 fusion. eLIFE. 2021 | |
dc.identifier.uri | http://hdl.handle.net/10852/90430 | |
dc.description.abstract | Precision CRISPR gene editing relies on the cellular homology-directed DNA repair (HDR) to introduce custom DNA sequences to target sites. The HDR editing efficiency varies between cell types and genomic sites, and the sources of this variation are incompletely understood. Here, we have studied the effect of 450 DNA repair protein - Cas9 fusions on CRISPR genome editing outcomes. We find the majority of fusions to improve precision genome editing only modestly in a locus- and cell-type specific manner. We identify Cas9-POLD3 fusion that enhances editing by speeding up the initiation of DNA repair. We conclude that while DNA repair protein fusions to Cas9 can improve HDR CRISPR editing, most need to be optimized to the cell type and genomic site, highlighting the diversity of factors contributing to locus-specific genome editing outcomes. | |
dc.language | EN | |
dc.publisher | eLife Sciences Publications Ltd | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Rapid genome editing by CRISPR-Cas9-POLD3 fusion | |
dc.type | Journal article | |
dc.creator.author | Reint, Ganna | |
dc.creator.author | Li, Zhuokun | |
dc.creator.author | Labun, Kornel | |
dc.creator.author | Keskitalo, Salla | |
dc.creator.author | Soppa, Inkeri | |
dc.creator.author | Mamia, Katariina Aino Inkeri | |
dc.creator.author | Tolo, Eero | |
dc.creator.author | Szymanska, Monika | |
dc.creator.author | Meza-Zepeda, Leonardo A. | |
dc.creator.author | Lorenz, Susanne | |
dc.creator.author | Cieslar-Pobuda, Artur Grzegorz | |
dc.creator.author | Hu, Xian | |
dc.creator.author | Bodin, Diana L | |
dc.creator.author | Staerk, Judith | |
dc.creator.author | Valen, Eivind Dale | |
dc.creator.author | Schmierer, Bernhard | |
dc.creator.author | Varjosalo, Markku | |
dc.creator.author | Taipale, Jussi | |
dc.creator.author | Haapaniemi, Emma Maria | |
cristin.unitcode | 185,57,18,0 | |
cristin.unitname | Emma Haapaniemi Group - precision pediatrics and gene editing | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 2 | |
dc.identifier.cristin | 1975785 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=eLIFE&rft.volume=&rft.spage=&rft.date=2021 | |
dc.identifier.jtitle | eLIFE | |
dc.identifier.volume | 10 | |
dc.identifier.doi | https://doi.org/10.7554/eLife.75415 | |
dc.identifier.urn | URN:NBN:no-93022 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 2050-084X | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/90430/5/elife-75415-v2.pdf | |
dc.type.version | PublishedVersion | |
dc.relation.project | HSØ/279922 | |
dc.relation.project | KF/190290 | |
dc.relation.project | NFR/187615 | |