dc.date.accessioned | 2022-01-31T18:46:59Z | |
dc.date.available | 2022-01-31T18:46:59Z | |
dc.date.created | 2021-12-07T13:52:30Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Svendsen, Henrik Aasrum Meling, Torstein Ragnar Nygaard, Vigdis Waagene, Stein Russnes, Hege Elisabeth Giercksky Juell, Siri Rogne, Siril Gudmundson Pahnke, Jens Helseth, Eirik Fodstad, Øystein Mælandsmo, Gunhild Mari . Novel human melanoma brain metastasis models in athymic nude fox1<sup>nu</sup> mice: Site-specific metastasis patterns reflecting their clinical origin. Cancer Medicine. 2021, 10(23), 8604-8613 | |
dc.identifier.uri | http://hdl.handle.net/10852/90327 | |
dc.description.abstract | Background
Malignant melanomas frequently metastasize to the brain, but metastases in the cerebellum are underrepresented compared with metastases in the cerebrum.
Methods
We established animal models by injecting intracardially in athymic nude fox1nu mice two human melanoma cell lines, originating from a cerebral metastasis (HM19) and a cerebellar metastasis (HM86).
Results
Using magnetic resonance imaging (MRI), metastases were first detected after a mean of 34.5 days. Mean survival time was 59.6 days for the mice in the HM86 group and significantly shorter (43.7 days) for HM19-injected animals (p < 0.001). In the HM86 group, the first detectable metastasis was located in the cerebellum in 15/55 (29%) mice compared with none in the HM19 group (p < 0.001). At sacrifice, cerebellar metastases were found in 34/55 (63%) HM86-injected mice compared with 1/53 (2%) in the HM19-injected (p < 0.001) mice. At that time, all mice in both groups had detectable metastases in the cerebrum. Comparing macroscopic and histologic appearances of the brain metastases with their clinical counterparts, the cell line-based tumors had kept their original morphologic characteristics.
Conclusions
The present work demonstrates that human brain-metastatic melanoma cells injected intracardially in mice had retained inherent characteristics also in reproducing interaction with subtle microenvironmental brain tissue compartment-specific features. The models offer new possibilities for investigating tumor- and host-associated factors involved in determining tissue specificity of brain metastasis. | |
dc.language | EN | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Novel human melanoma brain metastasis models in athymic nude fox1<sup>nu</sup> mice: Site-specific metastasis patterns reflecting their clinical origin | |
dc.type | Journal article | |
dc.creator.author | Svendsen, Henrik Aasrum | |
dc.creator.author | Meling, Torstein Ragnar | |
dc.creator.author | Nygaard, Vigdis | |
dc.creator.author | Waagene, Stein | |
dc.creator.author | Russnes, Hege Elisabeth Giercksky | |
dc.creator.author | Juell, Siri | |
dc.creator.author | Rogne, Siril Gudmundson | |
dc.creator.author | Pahnke, Jens | |
dc.creator.author | Helseth, Eirik | |
dc.creator.author | Fodstad, Øystein | |
dc.creator.author | Mælandsmo, Gunhild Mari | |
cristin.unitcode | 185,53,42,11 | |
cristin.unitname | Nevrokirurgisk avdeling | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 1965630 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Cancer Medicine&rft.volume=10&rft.spage=8604&rft.date=2021 | |
dc.identifier.jtitle | Cancer Medicine | |
dc.identifier.volume | 10 | |
dc.identifier.issue | 23 | |
dc.identifier.startpage | 8604 | |
dc.identifier.endpage | 8613 | |
dc.identifier.doi | https://doi.org/10.1002/cam4.4334 | |
dc.identifier.urn | URN:NBN:no-92931 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 2045-7634 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/90327/2/article12484.pdf | |
dc.type.version | PublishedVersion | |