dc.date.accessioned | 2022-01-29T16:25:22Z | |
dc.date.available | 2022-01-29T16:25:22Z | |
dc.date.created | 2021-11-23T14:56:08Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Ahmadi, Arash Till, Katharina Backe, Paul Hoff Blicher, Pernille Diekmann, Robin Schüttpelz, Mark Glette, Kyrre Tørresen, Jim Bjørås, Magnar Rowe, Alexander D. Dalhus, Bjørn . Non-flipping DNA glycosylase AlkD scans DNA without formation of a stable interrogation complex. Communications Biology. 2021 | |
dc.identifier.uri | http://hdl.handle.net/10852/90297 | |
dc.description.abstract | Abstract The multi-step base excision repair (BER) pathway is initiated by a set of enzymes, known as DNA glycosylases, able to scan DNA and detect modified bases among a vast number of normal bases. While DNA glycosylases in the BER pathway generally bend the DNA and flip damaged bases into lesion specific pockets, the HEAT-like repeat DNA glycosylase AlkD detects and excises bases without sequestering the base from the DNA helix. We show by single-molecule tracking experiments that AlkD scans DNA without forming a stable interrogation complex. This contrasts with previously studied repair enzymes that need to flip bases into lesion-recognition pockets and form stable interrogation complexes. Moreover, we show by design of a loss-of-function mutant that the bimodality in scanning observed for the structural homologue AlkF is due to a key structural differentiator between AlkD and AlkF; a positively charged β-hairpin able to protrude into the major groove of DNA. | |
dc.language | EN | |
dc.publisher | Nature Portfolio | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Non-flipping DNA glycosylase AlkD scans DNA without formation of a stable interrogation complex | |
dc.type | Journal article | |
dc.creator.author | Ahmadi, Arash | |
dc.creator.author | Till, Katharina | |
dc.creator.author | Backe, Paul Hoff | |
dc.creator.author | Blicher, Pernille | |
dc.creator.author | Diekmann, Robin | |
dc.creator.author | Schüttpelz, Mark | |
dc.creator.author | Glette, Kyrre | |
dc.creator.author | Tørresen, Jim | |
dc.creator.author | Bjørås, Magnar | |
dc.creator.author | Rowe, Alexander D. | |
dc.creator.author | Dalhus, Bjørn | |
cristin.unitcode | 185,53,18,14 | |
cristin.unitname | Avdeling for medisinsk biokjemi | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 1957939 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Communications Biology&rft.volume=&rft.spage=&rft.date=2021 | |
dc.identifier.jtitle | Communications Biology | |
dc.identifier.volume | 4 | |
dc.identifier.issue | 1 | |
dc.identifier.doi | https://doi.org/10.1038/s42003-021-02400-x | |
dc.identifier.urn | URN:NBN:no-92899 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 2399-3642 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/90297/1/s42003-021-02400-x.pdf | |
dc.type.version | PublishedVersion | |
cristin.articleid | 876 | |