dc.date.accessioned | 2022-01-24T19:27:58Z | |
dc.date.available | 2022-01-24T19:27:58Z | |
dc.date.created | 2021-12-20T10:25:46Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Bakkalci, Deniz Jay, Amrita Rezaei, Azadeh Howard, Christopher A. Haugen, Håvard Jostein Pape, Judith Kishida, Shosei Kishida, Michiko Jell, Gavin Arnett, Timothy R. Fedele, Stefano Cheema, Umber . Bioengineering the ameloblastoma tumour to study its efect on bone nodule formation. Scientific Reports. 2021, 11(24088) | |
dc.identifier.uri | http://hdl.handle.net/10852/90037 | |
dc.description.abstract | Ameloblastoma is a benign, epithelial cancer of the jawbone, which causes bone resorption and disfigurement to patients affected. The interaction of ameloblastoma with its tumour stroma drives invasion and progression. We used stiff collagen matrices to engineer active bone forming stroma, to probe the interaction of ameloblastoma with its native tumour bone microenvironment. This bone-stroma was assessed by nano-CT, transmission electron microscopy (TEM), Raman spectroscopy and gene analysis. Furthermore, we investigated gene correlation between bone forming 3D bone stroma and ameloblastoma introduced 3D bone stroma. Ameloblastoma cells increased expression of MMP-2 and -9 and RANK temporally in 3D compared to 2D. Our 3D biomimetic model formed bone nodules of an average surface area of 0.1 mm2 and average height of 92.37 ± 7.96 μm over 21 days. We demonstrate a woven bone phenotype with distinct mineral and matrix components and increased expression of bone formation genes in our engineered bone. Introducing ameloblastoma to the bone stroma, completely inhibited bone formation, in a spatially specific manner. Multivariate gene analysis showed that ameloblastoma cells downregulate bone formation genes such as RUNX2. Through the development of a comprehensive bone stroma, we show that an ameloblastoma tumour mass prevents osteoblasts from forming new bone nodules and severely restricted the growth of existing bone nodules. We have identified potential pathways for this inhibition. More critically, we present novel findings on the interaction of stromal osteoblasts with ameloblastoma. | |
dc.language | EN | |
dc.publisher | Nature Portfolio | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Bioengineering the ameloblastoma tumour to study its efect on bone nodule formation | |
dc.type | Journal article | |
dc.creator.author | Bakkalci, Deniz | |
dc.creator.author | Jay, Amrita | |
dc.creator.author | Rezaei, Azadeh | |
dc.creator.author | Howard, Christopher A. | |
dc.creator.author | Haugen, Håvard Jostein | |
dc.creator.author | Pape, Judith | |
dc.creator.author | Kishida, Shosei | |
dc.creator.author | Kishida, Michiko | |
dc.creator.author | Jell, Gavin | |
dc.creator.author | Arnett, Timothy R. | |
dc.creator.author | Fedele, Stefano | |
dc.creator.author | Cheema, Umber | |
cristin.unitcode | 185,16,17,62 | |
cristin.unitname | Biomaterialer | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 1970411 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Scientific Reports&rft.volume=11&rft.spage=&rft.date=2021 | |
dc.identifier.jtitle | Scientific Reports | |
dc.identifier.volume | 11 | |
dc.identifier.issue | 1 | |
dc.identifier.doi | https://doi.org/10.1038/s41598-021-03484-5 | |
dc.identifier.urn | URN:NBN:no-92639 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 2045-2322 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/90037/1/s41598-021-03484-5.pdf | |
dc.type.version | PublishedVersion | |
cristin.articleid | 24088 | |