dc.date.accessioned | 2021-10-14T15:32:13Z | |
dc.date.available | 2021-10-14T15:32:13Z | |
dc.date.created | 2021-08-23T12:56:07Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Skogvold, Hanne Bendiksen Sandås, Elise Mørk Østeby, Anja Løkken, Camilla Rootwelt, Helge Rønning, Per Ola Wilson, Steven Ray Haakon Elgstøen, Katja Benedikte Prestø . Bridging the Polar and Hydrophobic Metabolome in Single-Run Untargeted Liquid Chromatography-Mass Spectrometry Dried Blood Spot Metabolomics for Clinical Purposes. Journal of Proteome Research. 2021, 20(8), 4010-4021 | |
dc.identifier.uri | http://hdl.handle.net/10852/88931 | |
dc.description.abstract | Dried blood spot (DBS) metabolite analysis is a central tool for the clinic, e.g., newborn screening. Instead of applying multiple analytical methods, a single liquid chromatography-mass spectrometry (LC–MS) method was developed for metabolites spanning from highly polar glucose to hydrophobic long-chain acylcarnitines. For liquid chromatography, a diphenyl column and a multi-linear solvent gradient operated at elevated flow rates allowed for an even-spread resolution of diverse metabolites. Injecting moderate volumes of DBS organic extracts directly, in contrast to evaporation and reconstitution, provided substantial increases in analyte recovery. Q Exactive MS settings were also tailored for sensitivity increases, and the method allowed for analyte retention time and peak area repeatabilities of 0.1–0.4 and 2–10%, respectively, for a wide polarity range of metabolites (log P −4.4 to 8.8). The method’s performance was suited for both untargeted analysis and targeted approaches evaluated in clinically relevant experiments. | |
dc.language | EN | |
dc.publisher | ACS Publications | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Bridging the Polar and Hydrophobic Metabolome in Single-Run Untargeted Liquid Chromatography-Mass Spectrometry Dried Blood Spot Metabolomics for Clinical Purposes | |
dc.type | Journal article | |
dc.creator.author | Skogvold, Hanne Bendiksen | |
dc.creator.author | Sandås, Elise Mørk | |
dc.creator.author | Østeby, Anja | |
dc.creator.author | Løkken, Camilla | |
dc.creator.author | Rootwelt, Helge | |
dc.creator.author | Rønning, Per Ola | |
dc.creator.author | Wilson, Steven Ray Haakon | |
dc.creator.author | Elgstøen, Katja Benedikte Prestø | |
cristin.unitcode | 185,15,12,63 | |
cristin.unitname | Seksjon for kjemisk livsvitenskap - biomolekyler, bio-inspirerte materialer og bioanalytisk kjemi | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 2 | |
dc.identifier.cristin | 1928022 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal of Proteome Research&rft.volume=20&rft.spage=4010&rft.date=2021 | |
dc.identifier.jtitle | Journal of Proteome Research | |
dc.identifier.volume | 20 | |
dc.identifier.issue | 8 | |
dc.identifier.startpage | 4010 | |
dc.identifier.endpage | 4021 | |
dc.identifier.doi | https://doi.org/10.1021/acs.jproteome.1c00326 | |
dc.identifier.urn | URN:NBN:no-91545 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 1535-3893 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/88931/2/acs.jproteome.1c00326.pdf | |
dc.type.version | PublishedVersion | |