dc.date.accessioned | 2021-04-12T19:11:44Z | |
dc.date.available | 2021-04-12T19:11:44Z | |
dc.date.created | 2020-08-21T14:52:26Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Kocere, Agnese Resseguier, Julien Wohlmann, Jens Skjeldal, Frode Miltzow Khan, Shanawaz Speth, Martin Dal, Nils-Jørgen Knudsen Ng, Matthew Yoke Wui Alonso Rodriguez, Noelia Scarpa, Edoardo Rizzello, Loris Battaglia, Giuseppe Griffiths, Gareth Wyn Fenaroli, Federico . Real-time imaging of polymersome nanoparticles in zebrafish embryos engrafted with melanoma cancer cells: Localization, toxicity and treatment analysis. EBioMedicine. 2020, 58, 1-12 | |
dc.identifier.uri | http://hdl.handle.net/10852/85168 | |
dc.description.abstract | Background
The developing zebrafish is an emerging tool in nanomedicine, allowing non-invasive live imaging of the whole animal at higher resolution than is possible in the more commonly used mouse models. In addition, several transgenic fish lines are available endowed with selected cell types expressing fluorescent proteins; this allows nanoparticles to be visualized together with host cells.
Methods
Here, we introduce the zebrafish neural tube as a robust injection site for cancer cells, excellently suited for high resolution imaging. We use light and electron microscopy to evaluate cancer growth and to follow the fate of intravenously injected nanoparticles.
Findings
Fluorescently labelled mouse melanoma B16 cells, when injected into this structure proliferated rapidly and stimulated angiogenesis of new vessels. In addition, macrophages, but not neutrophils, selectively accumulated in the tumour region. When injected intravenously, nanoparticles made of Cy5-labelled poly(ethylene glycol)-block-poly(2-(diisopropyl amino) ethyl methacrylate) (PEG-PDPA) selectively accumulated in the neural tube cancer region and were seen in individual cancer cells and tumour associated macrophages. Moreover, when doxorubicin was released from PEG-PDPA, in a pH dependant manner, these nanoparticles could strongly reduce toxicity and improve the treatment outcome compared to the free drug in zebrafish xenotransplanted with mouse melanoma B16 or human derived melanoma cells.
Interpretation
The zebrafish has the potential of becoming an important intermediate step, before the mouse model, for testing nanomedicines against patient-derived cancer cells. | |
dc.language | EN | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.title | Real-time imaging of polymersome nanoparticles in zebrafish embryos engrafted with melanoma cancer cells: Localization, toxicity and treatment analysis | |
dc.type | Journal article | |
dc.creator.author | Kocere, Agnese | |
dc.creator.author | Resseguier, Julien | |
dc.creator.author | Wohlmann, Jens | |
dc.creator.author | Skjeldal, Frode Miltzow | |
dc.creator.author | Khan, Shanawaz | |
dc.creator.author | Speth, Martin | |
dc.creator.author | Dal, Nils-Jørgen Knudsen | |
dc.creator.author | Ng, Matthew Yoke Wui | |
dc.creator.author | Alonso Rodriguez, Noelia | |
dc.creator.author | Scarpa, Edoardo | |
dc.creator.author | Rizzello, Loris | |
dc.creator.author | Battaglia, Giuseppe | |
dc.creator.author | Griffiths, Gareth Wyn | |
dc.creator.author | Fenaroli, Federico | |
cristin.unitcode | 185,15,29,0 | |
cristin.unitname | Institutt for biovitenskap | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 1824535 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=EBioMedicine&rft.volume=58&rft.spage=1&rft.date=2020 | |
dc.identifier.jtitle | EBioMedicine | |
dc.identifier.volume | 58 | |
dc.identifier.doi | https://doi.org/10.1016/j.ebiom.2020.102902 | |
dc.identifier.urn | URN:NBN:no-87821 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 2352-3964 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/85168/2/Real-time%2Bimaging%2Bof%2Bpolymersome%2Bnanoparticles-1-s2.0-S2352396420302772-main.pdf | |
dc.type.version | PublishedVersion | |
cristin.articleid | 102902 | |
dc.relation.project | NFR/275873 | |
dc.relation.project | NFR/273319 | |
dc.relation.project | KF/206204 | |