dc.date.accessioned | 2021-01-26T19:23:23Z | |
dc.date.available | 2021-01-26T19:23:23Z | |
dc.date.created | 2021-01-18T12:44:06Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Josifovska, Natasha Albert, Réka Nagymihaly, Richard Lytvynchuk, Lyubomyr Moe, Morten Carsten Kaarniranta, Kai Veréb, Zoltán Petrovski, Goran . Resveratrol as Inducer of Autophagy, Pro-Survival, and Anti-Inflammatory Stimuli in Cultured Human RPE Cells. International Journal of Molecular Sciences. 2020, 21(3), 1-12 | |
dc.identifier.uri | http://hdl.handle.net/10852/82644 | |
dc.description.abstract | Purpose: To investigate the mechanism by which resveratrol acts upon retinal pigment epithelial (RPE) cells and to characterize its effect upon autophagy, survival, and inflammation, with consequent implications to treatment for age-related macular degeneration (AMD). Methods: Cultured ARPE-19 cells were exposed to 10 and 50 μM resveratrol. Cell survival/death was determined by annexin-FITC/propidium iodide using flow cytometry, while autophagy was studied by detecting autophagic vacuoles formation (acridine orange and transmission electron microscopy), as well as LC3II/I ratio and p62 expression by Western blot. In addition, time-lapse confocal microscopy of a pDENDRA-LC3 expression vector was performed to detect autophagy in transfected ARPE-19 cells under the different treatment conditions. Inhibition of proteasomal and autophagy-lysosomal fusion was carried out by MG-132 and chloroquine, respectively, while induction of autophagy was achieved by rapamycin treatment. Detection of secreted cytokines by ARPE-19 cells using Human XL Cytokine Array was performed under oxidative stress (H2O2) and resveratrol treatments, respectively. Results: Resveratrol induced autophagy in ARPE-19 cells as determined by augmented presence of autophagic vacuoles, increased LC3II/I ratio and decreased p62 expression, as well as time-lapse confocal microscopy using pDENDRA-LC3 expression vector. Resveratrol acted similarly to proteasomal inhibition and downstream of mammalian target of rapamycin (mTOR), since upstream inhibition of autophagy by 3-methyladenine could not inhibit autophagy in ARPE-19 cells. Co-treatmeant by rapamycin and/or proteasome inhibition showed no additive effect upon autophagy induction. ARPE-19 cells treated by resveratrol showed lower cell death rate compared to untreated controls. Resveratrol induced a specific anti-inflammatory response in ARPE-19 cells. Conclusions: Resveratrol can induce autophagy, pro-survival, and anti-inflammatory stimuli in ARPE-19 cells, properties which could be plausible to formulate future treatment modalities for AMD. | |
dc.language | EN | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Resveratrol as Inducer of Autophagy, Pro-Survival, and Anti-Inflammatory Stimuli in Cultured Human RPE Cells | |
dc.type | Journal article | |
dc.creator.author | Josifovska, Natasha | |
dc.creator.author | Albert, Réka | |
dc.creator.author | Nagymihaly, Richard | |
dc.creator.author | Lytvynchuk, Lyubomyr | |
dc.creator.author | Moe, Morten Carsten | |
dc.creator.author | Kaarniranta, Kai | |
dc.creator.author | Veréb, Zoltán | |
dc.creator.author | Petrovski, Goran | |
cristin.unitcode | 185,53,43,11 | |
cristin.unitname | Øyeavdelingen | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 1873191 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=International Journal of Molecular Sciences&rft.volume=21&rft.spage=1&rft.date=2020 | |
dc.identifier.jtitle | International Journal of Molecular Sciences | |
dc.identifier.volume | 21 | |
dc.identifier.issue | 3 | |
dc.identifier.doi | https://doi.org/10.3390/ijms21030813 | |
dc.identifier.urn | URN:NBN:no-85489 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 1422-0067 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/82644/1/Resveratrol%2Bas%2BInducer%2Bof%2BAutophagy%252C%2BPro-Survival%252C.pdf | |
dc.type.version | PublishedVersion | |
cristin.articleid | 813 | |