dc.date.accessioned | 2020-11-06T19:01:49Z | |
dc.date.available | 2020-11-06T19:01:49Z | |
dc.date.created | 2020-11-03T13:49:40Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Søland, Tine Merete Bjerkli, Inger-Heidi Georgsen, Jeanette B Schreurs, Olaf Joseph Franciscus Jebsen, Peter Wilhelm Laurvik, Helene Sapkota, Dipak . High-risk human papilloma virus was not detected in a Norwegian cohort of oral squamous cell carcinoma of the mobile tongue. Clinical and Experimental Dental Research. 2020 | |
dc.identifier.uri | http://hdl.handle.net/10852/80883 | |
dc.description.abstract | Abstract Objectives: The presence of and the causative role of high-risk human papilloma virus (HPV) is a subject of controversy in oral squamous cell carcinoma (OSCC). The disagreement can be related to the misclassification of OSCC as oropharyngeal squamous cell carcinoma and/or lack of standard detection methods. This study aimed to examine the presence of transcriptionally active high-risk HPV in a homogenous Norwegian cohort of primary and second primary OSCC of the mobile tongue (oral tongue squamous cell carcinoma—OTSCC). Methods: Tissue microarrays containing formalin-fixed and paraffin-embedded cores of 146 OTSCC from the anterior 2/3 of the tongue (n = 128 primary and n = 18 second primary) from a multicentric Norwegian cohort were examined for the presence of high-risk HPV by DNA- and RNA-in situ hybridization (ISH) assays and p16 immunohistochemistry. Results: Transcriptionally active HPV (E6/E7 mRNA) was not identified in any of the OTSCC specimens. In parallel, no tumors were positive for HPV by DNA ISH. Although, 61 (42%) OTSCC demonstrated p16 positivity with varying staining intensity and subcellular localization, only two cases demonstrated strong and uniform p16-staining (both cytoplasmic and nuclear) in >70% of cancer cells. The absence of transcriptionally active high-risk HPV in this cohort of OTSCC indicates that high-risk HPV is an unlikely causative factor in the present material. | |
dc.language | EN | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | High-risk human papilloma virus was not detected in a Norwegian cohort of oral squamous cell carcinoma of the mobile tongue | |
dc.type | Journal article | |
dc.creator.author | Søland, Tine Merete | |
dc.creator.author | Bjerkli, Inger-Heidi | |
dc.creator.author | Georgsen, Jeanette B | |
dc.creator.author | Schreurs, Olaf Joseph Franciscus | |
dc.creator.author | Jebsen, Peter Wilhelm | |
dc.creator.author | Laurvik, Helene | |
dc.creator.author | Sapkota, Dipak | |
cristin.unitcode | 185,16,15,0 | |
cristin.unitname | Institutt for oral biologi | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 1844535 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Clinical and Experimental Dental Research&rft.volume=&rft.spage=&rft.date=2020 | |
dc.identifier.jtitle | Clinical and Experimental Dental Research | |
dc.identifier.doi | https://doi.org/10.1002/cre2.342 | |
dc.identifier.urn | URN:NBN:no-83966 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 2057-4347 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/80883/1/HPV%2Bartikkel%2BTine.pdf | |
dc.type.version | PublishedVersion | |
cristin.articleid | cre2.342 | |