dc.date.accessioned | 2020-10-26T18:55:18Z | |
dc.date.available | 2020-10-26T18:55:18Z | |
dc.date.created | 2020-10-15T17:22:44Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Foss, Amanda J. Miles, Christopher O. Wilkins, Alistair Lawrence Rise, Frode Trovik, Kristian Wiedicke Cieslik, Kamil Aubel, Mark T. . Analysis of total microcystins and nodularins by oxidative cleavage of their ADMAdda, DMAdda, and Adda moieties. Analytica Chimica Acta. 2020, 6 | |
dc.identifier.uri | http://hdl.handle.net/10852/80746 | |
dc.description.abstract | Microcystins (MCs) and nodularins (NODs) exhibit high structural variability, including modifications of the Adda (3S-amino-9S-methoxy-2S,6,8S-trimethyl-10-phenyldeca-4E,6E-dienoic acid) moiety. Variations include 9-O-desmethylAdda (DMAdda) and 9-O-acetylDMAdda (ADMAdda) which, unless targeted, may go undetected. Therefore, reference standards were prepared of [ADMAdda5]MCs and [DMAdda5]MCs, which were analyzed using multiple approaches. The cross-reactivities of the [DMAdda5]- and [ADMAdda5]MC standards were similar to that of MC-LR when analyzed with a protein phosphatase 2A (PP2A) inhibition assay, but were <0.25% when analyzed with an Adda enzyme-linked immunosorbent assay (ELISA). Oxidative cleavage experiments identified compounds that could be used in the analysis of total MCs/NODs in a similar fashion to the 2R-methyl-3S-methoxy-4-phenylbutanoic acid (MMPB) technique. Products from oxidative cleavage of both the 4,5- and 6,7-ene of Adda, DMAdda and ADMAdda were observed, and three oxidation products, one from each Adda variant, were chosen for analysis and applied to three field samples and a Nostoc culture. Results from the oxidative cleavage method for total Adda, DMAdda, and ADMAdda were similar to those from the Adda-ELISA, PP2A inhibition, and LC-MS/MS analyses, except for the Nostoc culture where the Adda-ELISA greatly underestimated microcystin levels. This oxidative cleavage method can be used for routine analysis of field samples and to assess the presence of the rarely reported, but toxic, DMAdda/ADMAdda-containing MCs and NODs. | en_US |
dc.language | EN | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Analysis of total microcystins and nodularins by oxidative cleavage of their ADMAdda, DMAdda, and Adda moieties | en_US |
dc.type | Journal article | en_US |
dc.creator.author | Foss, Amanda J. | |
dc.creator.author | Miles, Christopher O. | |
dc.creator.author | Wilkins, Alistair Lawrence | |
dc.creator.author | Rise, Frode | |
dc.creator.author | Trovik, Kristian Wiedicke | |
dc.creator.author | Cieslik, Kamil | |
dc.creator.author | Aubel, Mark T. | |
cristin.unitcode | 185,15,12,57 | |
cristin.unitname | Organisk kjemi | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 1839956 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Analytica Chimica Acta&rft.volume=6&rft.spage=&rft.date=2020 | |
dc.identifier.jtitle | Analytica Chimica Acta | |
dc.identifier.volume | 6 | |
dc.identifier.pagecount | 15 | |
dc.identifier.doi | https://doi.org/10.1016/j.acax.2020.100060 | |
dc.identifier.urn | URN:NBN:no-83834 | |
dc.type.document | Tidsskriftartikkel | en_US |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 0003-2670 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/80746/2/1-s2.0-S2590134620300220-main.pdf | |
dc.type.version | PublishedVersion | |
cristin.articleid | 100060 | |