dc.date.accessioned | 2020-09-29T18:53:44Z | |
dc.date.available | 2020-09-29T18:53:44Z | |
dc.date.created | 2020-09-25T17:42:39Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Rørvik, Gro Herredsvela Liskiewicz, Krystyna Anna Kryuchkov, Fedor Naemi, Ali-Oddin aasheim, Hans-Christian Petersen, Fernanda Cristina Küntziger, Thomas M. Simm, Roger . Cyclic Di-adenosine Monophosphate Regulates Metabolism and Growth in the Oral Commensal Streptococcus mitis. Microorganisms. 2020 | |
dc.identifier.uri | http://hdl.handle.net/10852/80164 | |
dc.description.abstract | Cyclic di-adenosine monophosphate (c-di-AMP) has emerged as an important bacterial signaling molecule that functions both as an intracellular second messenger in bacterial cells and an extracellular ligand involved in bacteria-host cross-talk. In this study, we identify and characterize proteins involved in controlling the c-di-AMP concentration in the oral commensal and opportunistic pathogen Streptococcusmitis (S. mitis). We identified three known types of c-di-AMP turnover proteins in the genome of S. mitis CCUG31611: a CdaA-type diadenylate cyclase as well as GdpP-, and DhhP-type phosphodiesterases. Biochemical analyses of purified proteins demonstrated that CdaA synthesizes c-di-AMP from ATP whereas both phosphodiesterases can utilize c-di-AMP as well as the intermediary metabolite of c-di-AMP hydrolysis 5′-phosphadenylyl-adenosine (pApA) as substrate to generate AMP, albeit at different catalytic efficiency. Using deletion mutants of each of the genes encoding c-di-AMP turnover proteins, we show by high resolution MS/MS that the intracellular concentration of c-di-AMP is increased in deletion mutants of the phosphodiesterases and non-detectable in the cdaA-mutant. We also detected pApA in mutants of the DhhP-type phosphodiesterase. Low and high levels of c-di-AMP were associated with longer and shorter chains of S. mitis, respectively indicating a role in regulation of cell division. The deletion mutant of the DhhP-type phosphodiesterase displayed slow growth and reduced rate of glucose metabolism. | |
dc.language | EN | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Cyclic Di-adenosine Monophosphate Regulates Metabolism and Growth in the Oral Commensal Streptococcus mitis | |
dc.type | Journal article | |
dc.creator.author | Rørvik, Gro Herredsvela | |
dc.creator.author | Liskiewicz, Krystyna Anna | |
dc.creator.author | Kryuchkov, Fedor | |
dc.creator.author | Naemi, Ali-Oddin | |
dc.creator.author | Aasheim, Hans-Christian | |
dc.creator.author | Petersen, Fernanda Cristina | |
dc.creator.author | Küntziger, Thomas M. | |
dc.creator.author | Simm, Roger | |
cristin.unitcode | 185,16,15,0 | |
cristin.unitname | Institutt for oral biologi | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 1833668 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Microorganisms&rft.volume=&rft.spage=&rft.date=2020 | |
dc.identifier.jtitle | Microorganisms | |
dc.identifier.volume | 8 | |
dc.identifier.issue | 9 | |
dc.identifier.doi | https://doi.org/10.3390/microorganisms8091269 | |
dc.identifier.urn | URN:NBN:no-83257 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 2076-2607 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/80164/2/microorganisms-08-01269-v2.pdf | |
dc.type.version | PublishedVersion | |
cristin.articleid | 1269 | |