dc.date.accessioned | 2020-07-10T17:56:03Z | |
dc.date.available | 2020-07-10T17:56:03Z | |
dc.date.created | 2020-01-14T08:44:03Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Hamilton, Brett R. Marshall, David L. Casewell, Nicholas R. Harrison, Robert A. Blanksby, Stephen J. Undheim, Eivind Andreas Baste . Mapping enzyme activity on tissue by functional mass spectrometry imaging. Angewandte Chemie International Edition. 2019, 59 | |
dc.identifier.uri | http://hdl.handle.net/10852/77746 | |
dc.description.abstract | Enzymes are central components of most physiological processes, and are consequently implicated in various pathologies. High‐resolution maps of enzyme activity within tissues therefore represent powerful tools for elucidating enzymatic functions in health and disease. Here, we present a novel mass spectrometry imaging (MSI) method for assaying the spatial distribution of enzymatic activity directly from tissue. MSI analysis of tissue sections exposed to phospholipid substrates produced high‐resolution maps of phospholipase activity and specificity, which could subsequently be compared to histological images of the same section. Functional MSI thus represents a new and generalisable method for imaging biological activity in situ. | |
dc.language | EN | |
dc.publisher | Wiley - VCH Verlag GmbH | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Mapping enzyme activity on tissue by functional mass spectrometry imaging | |
dc.type | Journal article | |
dc.creator.author | Hamilton, Brett R. | |
dc.creator.author | Marshall, David L. | |
dc.creator.author | Casewell, Nicholas R. | |
dc.creator.author | Harrison, Robert A. | |
dc.creator.author | Blanksby, Stephen J. | |
dc.creator.author | Undheim, Eivind Andreas Baste | |
cristin.unitcode | 185,15,29,50 | |
cristin.unitname | Centre for Ecological and Evolutionary Synthesis | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 2 | |
dc.identifier.cristin | 1771961 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Angewandte Chemie International Edition&rft.volume=59&rft.spage=&rft.date=2019 | |
dc.identifier.jtitle | Angewandte Chemie International Edition | |
dc.identifier.volume | 59 | |
dc.identifier.issue | 10 | |
dc.identifier.startpage | 3855 | |
dc.identifier.endpage | 3858 | |
dc.identifier.pagecount | 4 | |
dc.identifier.doi | https://doi.org/10.1002/anie.201911390 | |
dc.identifier.urn | URN:NBN:no-80908 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 1433-7851 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/77746/1/Mapping%2Benzyme%2Bactivity-anie.201911390.pdf | |
dc.type.version | PublishedVersion | |