dc.date.accessioned | 2020-06-24T18:35:10Z | |
dc.date.available | 2020-06-24T18:35:10Z | |
dc.date.created | 2020-01-06T11:43:13Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | Solheim, Stian Hutchinson, Samantha A Lundanes, Elsa Wilson, Steven Ray Haakon Thorne, James L Røberg-Larsen, Hanne . Fast liquid chromatography-mass spectrometry reveals side chain oxysterol heterogeneity in breast cancer tumour samples. Journal of Steroid Biochemistry and Molecular Biology. 2019, 192 | |
dc.identifier.uri | http://hdl.handle.net/10852/77198 | |
dc.description.abstract | Oxysterols can contribute to proliferation of breast cancer through activation of the Estrogen Receptors, and to metastasis through activation of the Liver X Receptors. Endogenous levels of both esterified and free sidechain-hydroxylated oxysterols were examined in breast cancer tumours from Estrogen Receptor positive and negative breast tumours, using a novel fast liquid chromatography tandem mass spectrometry method. Multiple aliquots of five milligram samples of 22 tumours were analysed for oxysterol content to assess intra- and inter-tumour variation. Derivatization was performed with Girard T reagent (with and without alkaline hydrolysis) and sample clean-up was performed using a robust automatic on-line column switching system (“AFFL”). Oxysterols were separated isocratically on a 2.1 mm inner diameter column packed with ACE SuperPhenylHexyl core shell particles using a mobile phase consisting of 0.1% formic acid in H2O/methanol/acetonitrile (57/10/33, v/v/v) followed by a wash out step (0.1% formic acid in methanol/acetonitrile, 50/50, v/v). The total analysis time, including sample clean-up and column reconditioning, was 8 min (80% time reduction compared to other on-line systems). Analysis revealed large intra-tumour variations of sidechain oxysterols, resulting in no significant differences in endogenous oxysterols levels between Estrogen Receptor positive and Estrogen Receptor negative breast cancers. However, a correlation between esterified and free 27-hydroxycholesterol was observed. The same correlation was not observed for 24S-hydroxycholesterol or 25-hydroxycholesterol. The oxysterol heterogeneity of tumour tissue is a critical factor when assessing the role of these lipids in cancer. | |
dc.language | EN | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Fast liquid chromatography-mass spectrometry reveals side chain oxysterol heterogeneity in breast cancer tumour samples | |
dc.type | Journal article | |
dc.creator.author | Solheim, Stian | |
dc.creator.author | Hutchinson, Samantha A | |
dc.creator.author | Lundanes, Elsa | |
dc.creator.author | Wilson, Steven Ray Haakon | |
dc.creator.author | Thorne, James L | |
dc.creator.author | Røberg-Larsen, Hanne | |
cristin.unitcode | 185,15,12,0 | |
cristin.unitname | Kjemisk institutt | |
cristin.ispublished | true | |
cristin.fulltext | postprint | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 1766724 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal of Steroid Biochemistry and Molecular Biology&rft.volume=192&rft.spage=&rft.date=2019 | |
dc.identifier.jtitle | Journal of Steroid Biochemistry and Molecular Biology | |
dc.identifier.volume | 192 | |
dc.identifier.doi | https://doi.org/10.1016/j.jsbmb.2019.02.004 | |
dc.identifier.urn | URN:NBN:no-80288 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 0960-0760 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/77198/5/1-s2.0-S0960076018307544-main.pdf | |
dc.type.version | PublishedVersion | |
cristin.articleid | 105309 | |