dc.date.accessioned | 2020-06-17T18:33:25Z | |
dc.date.available | 2020-06-17T18:33:25Z | |
dc.date.created | 2019-06-27T17:53:33Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | Cho, Tiffany Bott, Debbie Ahmed, Shaimaa Hutin, David Gomez, Alvin Tamblyn, Laura Zhou, Angela C. Watts, Tania H. Grant, Denis M Matthews, Jason . 3-Methylcholanthrene Induces Chylous Ascites in TCDD-Inducible Poly-ADP-Ribose Polymerase (Tiparp) Knockout Mice. International Journal of Molecular Sciences. 2019, 20(9), 1-15 | |
dc.identifier.uri | http://hdl.handle.net/10852/77032 | |
dc.description.abstract | TCDD-inducible poly-ADP-ribose polymerase (TIPARP) is an aryl hydrocarbon receptor (AHR) target gene that functions as part of a negative feedback loop to repress AHR activity. Tiparp−/− mice exhibit increased sensitivity to the toxicological effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), including lethal wasting syndrome. However, it is not known whether Tiparp−/− mice also exhibit increased sensitivity to other AHR ligands. In this study, we treated male Tiparp−/− or wild type (WT) mice with a single injection of 100 mg/kg 3-methylcholanthrene (3MC). Consistent with TIPARP’s role as a repressor of AHR signaling, 3MC-treated Tiparp−/− mice exhibited increased hepatic Cyp1a1 and Cyp1b1 levels compared with WT mice. No 3MC-treated Tiparp−/− mice survived beyond day 16 and the mice exhibited chylous ascites characterized by an accumulation of fluid in the peritoneal cavity. All WT mice survived the 30-day treatment and showed no signs of fluid accumulation. Treated Tiparp−/− mice also exhibited a transient and mild hepatotoxicity with inflammation. 3MC-treated WT, but not Tiparp−/− mice, developed mild hepatic steatosis. Lipid deposits accumulated on the surface of the liver and other abdominal organs in the 3MC-Tiparp−/− mice. Our study reveals that Tiparp−/− mice have increased sensitivity to 3MC-induced liver toxicity, but unlike with TCDD, lethality is due to chylous ascites rather than wasting syndrome. | |
dc.language | EN | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | 3-Methylcholanthrene Induces Chylous Ascites in TCDD-Inducible Poly-ADP-Ribose Polymerase (Tiparp) Knockout Mice | |
dc.type | Journal article | |
dc.creator.author | Cho, Tiffany | |
dc.creator.author | Bott, Debbie | |
dc.creator.author | Ahmed, Shaimaa | |
dc.creator.author | Hutin, David | |
dc.creator.author | Gomez, Alvin | |
dc.creator.author | Tamblyn, Laura | |
dc.creator.author | Zhou, Angela C. | |
dc.creator.author | Watts, Tania H. | |
dc.creator.author | Grant, Denis M | |
dc.creator.author | Matthews, Jason | |
cristin.unitcode | 185,51,13,46 | |
cristin.unitname | Molekylær toksikologi | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 1708441 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=International Journal of Molecular Sciences&rft.volume=20&rft.spage=1&rft.date=2019 | |
dc.identifier.jtitle | International Journal of Molecular Sciences | |
dc.identifier.volume | 20 | |
dc.identifier.issue | 9 | |
dc.identifier.doi | https://doi.org/10.3390/ijms20092312 | |
dc.identifier.urn | URN:NBN:no-80127 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 1422-0067 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/77032/1/ijms-20-02312.pdf | |
dc.type.version | PublishedVersion | |
cristin.articleid | 2312 | |