dc.date.accessioned | 2020-05-06T19:49:54Z | |
dc.date.available | 2020-05-06T19:49:54Z | |
dc.date.created | 2019-09-16T11:16:32Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | Prikrylova, Terezia Robertson, Julia Ferrucci, Francesca Konorska, Dorota Joanna Aanes, Håvard Manaf, Adeel Zhang, Beibei Vågbø, Cathrine Broberg Kusnierczyk, Anna Gilljam, Karin Margaretha Løvkvam-Køster, Caroline Otterlei, Marit Dahl, John Arne Enserink, Jorrit Klungland, Arne Robertson, Adam Brian . 5-hydroxymethylcytosine marks mammalian origins acting as a barrier to replication. Scientific Reports. 2019, 9:11065, 1-16 | |
dc.identifier.uri | http://hdl.handle.net/10852/75200 | |
dc.description.abstract | In most mammalian cells, DNA replication occurs once, and only once between cell divisions. Replication initiation is a highly regulated process with redundant mechanisms that prevent errant initiation events. In lower eukaryotes, replication is initiated from a defined consensus sequence, whereas a consensus sequence delineating mammalian origin of replication has not been identified. Here we show that 5-hydroxymethylcytosine (5hmC) is present at mammalian replication origins. Our data support the hypothesis that 5hmC has a role in cell cycle regulation. We show that 5hmC level is inversely proportional to proliferation; indeed, 5hmC negatively influences cell division by increasing the time a cell resides in G1. Our data suggest that 5hmC recruits replication-licensing factors, then is removed prior to or during origin firing. Later we propose that TET2, the enzyme catalyzing 5mC to 5hmC conversion, acts as barrier to rereplication. In a broader context, our results significantly advance the understating of 5hmC involvement in cell proliferation and disease states. | |
dc.language | EN | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | 5-hydroxymethylcytosine marks mammalian origins acting as a barrier to replication | |
dc.type | Journal article | |
dc.creator.author | Prikrylova, Terezia | |
dc.creator.author | Robertson, Julia | |
dc.creator.author | Ferrucci, Francesca | |
dc.creator.author | Konorska, Dorota Joanna | |
dc.creator.author | Aanes, Håvard | |
dc.creator.author | Manaf, Adeel | |
dc.creator.author | Zhang, Beibei | |
dc.creator.author | Vågbø, Cathrine Broberg | |
dc.creator.author | Kusnierczyk, Anna | |
dc.creator.author | Gilljam, Karin Margaretha | |
dc.creator.author | Løvkvam-Køster, Caroline | |
dc.creator.author | Otterlei, Marit | |
dc.creator.author | Dahl, John Arne | |
dc.creator.author | Enserink, Jorrit | |
dc.creator.author | Klungland, Arne | |
dc.creator.author | Robertson, Adam Brian | |
cristin.unitcode | 185,51,0,0 | |
cristin.unitname | Institutt for medisinske basalfag | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 1725022 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Scientific Reports&rft.volume=9:11065&rft.spage=1&rft.date=2019 | |
dc.identifier.jtitle | Scientific Reports | |
dc.identifier.volume | 9 | |
dc.identifier.issue | 1 | |
dc.identifier.doi | https://doi.org/10.1038/s41598-019-47528-3 | |
dc.identifier.urn | URN:NBN:no-78281 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 2045-2322 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/75200/1/1725022.pdf | |
dc.type.version | PublishedVersion | |
cristin.articleid | 11065 | |
dc.relation.project | NFR/262652 | |
dc.relation.project | NFR/32182 | |
dc.relation.project | HSØ/39720 | |
dc.relation.project | HSØ/39641 | |