dc.date.accessioned | 2020-05-05T18:46:24Z | |
dc.date.available | 2020-05-05T18:46:24Z | |
dc.date.created | 2019-08-06T10:43:50Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | Stiksrud, Birgitte Aass, Hans Christian Dalsbotten Lorvik, Kristina Berg Ueland, Thor Trøseid, Marius Dyrhol-Riise, Anne Ma . Activated dendritic cells and monocytes in HIV immunological nonresponders: HIV-induced interferon-inducible protein-10 correlates with low future CD4+ recovery. AIDS (London). 2019, 33(7), 1117-1129 | |
dc.identifier.uri | http://hdl.handle.net/10852/75128 | |
dc.description.abstract | Objective:
To explore monocyte and dendritic cell immune responses, and their association with future CD4+ gain in treated HIV patients with suboptimal CD4+ recovery.
Design:
A cross-sectional study of HIV-infected, virally suppressed individuals on antiretroviral therapy for at least 24 months; 41 immunological nonresponders (INRs) (CD4+ cell count <400 cells/μl) and 26 immunological responders (CD4+ cell count >600 cells/μl). Ten HIV-infected antiretroviral therapy-naive and 10 HIV-negative healthy persons served as controls. CD4+ cell counts were registered after median 2.4 and 4.7 years.
Methods:
Monocyte, dendritic-cell and T-cell activation and regulatory T cells (Tregs) were analyzed by flow cytometry. In INR and immunological responder subgroups matched on age and nadir CD4+ cell count, upregulation of interferon-inducible protein-10 (IP-10) and indoleamine 2,3-dioxygenase in monocytes and dendritic cells and cytokines in cell supernatants were measured in vitro in peripheral blood mononuclear cells stimulated with aldrithiol-2-inactivated HIV-1.
Results:
The INR group displayed higher spontaneous activation of both monocytes (HLA-DR+) and myeloid and plasmacytoid dendritic cells (HLA-DR+, CD83+ and CD86+) compared with immunological responders, and this was associated with increased T-cell activation (CD38+HLA-DR+), an effector memory T-cell phenotype and activated Tregs. The IP-10 response in monocytes after in-vitro HIV stimulation was negatively associated with prospective CD4+ gain. IP-10, indoleamine 2,3-dioxygenase and cytokines levels were comparable between the groups, but inversely correlated with activated Tregs in INRs.
Conclusion:
HIV-infected individuals with suboptimal immune recovery demonstrated more activated monocytes and in particular dendritic cells, compared with patients with acceptable CD4+ gain. A high level of HIV-specific IP-10 expression in monocytes may be predictive of future CD4+ recovery. | |
dc.language | EN | |
dc.publisher | Lippincott Williams & Wilkins | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.title | Activated dendritic cells and monocytes in HIV immunological nonresponders: HIV-induced interferon-inducible protein-10 correlates with low future CD4+ recovery | |
dc.type | Journal article | |
dc.creator.author | Stiksrud, Birgitte | |
dc.creator.author | Aass, Hans Christian Dalsbotten | |
dc.creator.author | Lorvik, Kristina Berg | |
dc.creator.author | Ueland, Thor | |
dc.creator.author | Trøseid, Marius | |
dc.creator.author | Dyrhol-Riise, Anne Ma | |
cristin.unitcode | 185,53,11,14 | |
cristin.unitname | Infeksjonsmedisinsk avdeling | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.fulltext | postprint | |
cristin.qualitycode | 2 | |
dc.identifier.cristin | 1714251 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=AIDS (London)&rft.volume=33&rft.spage=1117&rft.date=2019 | |
dc.identifier.jtitle | AIDS (London) | |
dc.identifier.volume | 33 | |
dc.identifier.issue | 7 | |
dc.identifier.startpage | 1117 | |
dc.identifier.endpage | 1129 | |
dc.identifier.doi | https://doi.org/10.1097/QAD.0000000000002173 | |
dc.identifier.urn | URN:NBN:no-78220 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 0269-9370 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/75128/1/Activated_dendritic_cells_and_monocytes_in_HIV.2%2B%25283%2529.pdf | |
dc.type.version | PublishedVersion | |
dc.relation.project | NFR/144182 | |
dc.relation.project | NFR/187615 | |