dc.date.accessioned | 2020-03-13T14:57:46Z | |
dc.date.available | 2020-03-13T14:57:46Z | |
dc.date.issued | 2019 | |
dc.identifier.uri | http://hdl.handle.net/10852/74001 | |
dc.description.abstract | Spinal cord injury leads to a rapid and profound loss of skeletal muscle mass. Muscle atrophy consequently promotes metabolic disturbances and leads to increased risk of type 2 diabetes and cardiovascular disease. Prevention of such consequences requires a deeper understanding of underlying molecular and cellular changes, which promote muscle atrophy.
This thesis attempts to elucidate some of the mechanisms responsible for muscle atrophy induced by spinal cord injury; specifically, changes in the abundance of regulators of protein metabolism and enzymes responsible for oxidative stress homeostasis in skeletal muscle. Additionally, we examined the impact of spinal cord injury on the differentiation capacity of satellite cells, measured in vitro.
Our results suggest most profound changes in skeletal muscle within the first three months post-injury, including higher reactive oxygen species production, apoptosis, and protein turnover. Conversely, we show retained intrinsic satellite cell differentiation capacity, despite substantial changes within skeletal muscle. Collectively, the studies in this thesis encourage efforts to maintain protein metabolism balance and oxidative stress homeostasis during the early post-spinal cord injury phases, as well as rehabilitative interventions targeting satellite cell activation. | en_US |
dc.language.iso | en | en_US |
dc.relation.haspart | Paper I: Lundell LS, Savikj M, Kostovski E, Iversen PO, Zierath JR, Krook A, Chibalin AV, Widegren U. “Protein translation, proteolysis and autophagy in human skeletal muscle atrophy after spinal cord injury.” Acta Physiol 2018; 223:e13051. DOI: 10.1111/apha.13051. The article is not available in DUO due to publisher restrictions. The published version is available at: https://doi.org/10.1111/apha.13051 | |
dc.relation.haspart | Paper II: Savikj M, Kostovski E, Lundell LS, Iversen PO, Massart J, Widegren U. “Altered oxidative stress and antioxidant defences in skeletal muscle during the first year following spinal cord injury”. Published in: Physiological Reports, 2019, 7(16), pp 1-13. doi: 10.14814/phy2.14218. The article is included in the thesis. Also available in DUO: http://hdl.handle.net/10852/74000 | |
dc.relation.haspart | Paper III: Savikj M, Ruby MA, Kostovski E, Iversen PO, Zierath JR, Krook A, Widegren U. “Retained differentiation capacity of human skeletal muscle satellite cells from spinal cord-injured individuals.” Physiol Rep. 2018; 6:e13739. DOI: 10.14814/phy2.13739. The article is included in the thesis. Also available in DUO: http://urn.nb.no/URN:NBN:no-74636 | |
dc.relation.uri | https://doi.org/10.1111/apha.13051 | |
dc.relation.uri | http://hdl.handle.net/10852/74000 | |
dc.relation.uri | http://urn.nb.no/URN:NBN:no-74636 | |
dc.title | Cellular and molecular mechanisms of skeletal muscle atrophy after spinal cord injury | en_US |
dc.type | Doctoral thesis | en_US |
dc.creator.author | Savikj, Mladen | |
dc.identifier.urn | URN:NBN:no-77113 | |
dc.type.document | Doktoravhandling | en_US |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/74001/1/PhD-MladenSavikj-2019.pdf | |