dc.date.accessioned | 2019-12-10T19:33:42Z | |
dc.date.available | 2019-12-10T19:33:42Z | |
dc.date.created | 2018-08-04T14:55:36Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Reddy, Shiva Krogvold, Lars Martin, Charlton Holland, Rebecca Choi, Jaimin Woo, Hannah Wu, Fiona Dahl-Jørgensen, Knut . Distribution of IL-1β immunoreactive cells in pancreatic biopsies from living volunteers with new-onset type 1 diabetes: comparison with donors without diabetes and with longer duration of disease. Diabetologia. 2018, 61(6), 1362-1373 | |
dc.identifier.uri | http://hdl.handle.net/10852/71526 | |
dc.description.abstract | Aims/hypothesis
Although IL-1β is considered a key mediator of beta cell destruction, its cellular expression in islets during early type 1 diabetes remains unclear. We compared its expression in rare pancreatic biopsies from new-onset living volunteers with its expression in cadaveric pancreas sections from non-diabetic autoantibody-positive and -negative individuals and those with long-standing disease.
Methods
Pancreatic biopsy sections from six new-onset living volunteers (group 1) and cadaveric sections from 13 non-diabetic autoantibody-negative donors (group 2), four non-diabetic autoantibody-positive donors (group 3) and nine donors with diabetes of longer duration (0.25–12 years of disease; group 4) were triple-immunostained for IL-1β, insulin and glucagon. Intra- and peri-islet IL-1β-positive cells in insulin-positive and -negative islets and in random exocrine fields were enumerated.
Results
The mean number of IL-1β-positive cells per islet from each donor in peri- and intra-islet regions was <1.25 and <0.5, respectively. In all study groups, the percentage of islets with IL-1β cells in peri- and/or intra-islet regions was highly variable and ranged from 4.48% to 17.59% in group 1, 1.42% to 44.26% in group 2, 7.93% to 17.53% in group 3 and 3.85% to 42.86% in group 4, except in a single case where the value was 75%. In 25/32 donors, a higher percentage of islets showed IL-1β-positive cells in peri-islet than in intra-islet regions. In sections from diabetic donors (groups 1 and 4), a higher mean number of IL-1β-positive cells occurred in insulin-positive islets than in insulin-negative islets. In group 2, 70–90% of islets in 3/13 sections had weak-to-moderate IL-1β staining in alpha cells but staining was virtually absent or substantially reduced in the remaining groups. The mean number of exocrine IL-1β-positive cells in group 1 was lower than in the other groups.
Conclusions/interpretation
At onset of type 1 diabetes, the low number of islet-associated IL-1β-positive cells may be insufficient to elicit beta cell destruction. The variable expression in alpha cells in groups 2–4 suggests their cellular heterogeneity and probable physiological role. The significance of a higher but variable number of exocrine IL-1β-positive cells seen in non-diabetic individuals and those with long-term type 1 diabetes remains unclear. | |
dc.language | EN | |
dc.publisher | Springer Verlag | |
dc.title | Distribution of IL-1β immunoreactive cells in pancreatic biopsies from living volunteers with new-onset type 1 diabetes: comparison with donors without diabetes and with longer duration of disease | |
dc.type | Journal article | |
dc.creator.author | Reddy, Shiva | |
dc.creator.author | Krogvold, Lars | |
dc.creator.author | Martin, Charlton | |
dc.creator.author | Holland, Rebecca | |
dc.creator.author | Choi, Jaimin | |
dc.creator.author | Woo, Hannah | |
dc.creator.author | Wu, Fiona | |
dc.creator.author | Dahl-Jørgensen, Knut | |
cristin.unitcode | 185,16,17,56 | |
cristin.unitname | Avdeling for pedodonti og atferdsfag | |
cristin.ispublished | true | |
cristin.fulltext | postprint | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 1599751 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Diabetologia&rft.volume=61&rft.spage=1362&rft.date=2018 | |
dc.identifier.jtitle | Diabetologia | |
dc.identifier.volume | 61 | |
dc.identifier.issue | 6 | |
dc.identifier.startpage | 1362 | |
dc.identifier.endpage | 1373 | |
dc.identifier.doi | https://doi.org/10.1007/s00125-018-4600-8 | |
dc.identifier.urn | URN:NBN:no-74633 | |
dc.type.document | Tidsskriftartikkel | |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 0012-186X | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/71526/5/Reddy_et_al_2018_post-print.pdf | |
dc.type.version | AcceptedVersion | |