dc.date.accessioned | 2019-05-21T05:48:06Z | |
dc.date.available | 2019-05-21T05:48:06Z | |
dc.date.created | 2018-12-29T20:23:51Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Kolstad, Terje R Selnes Brink, Jonas van Den Macquaide, Niall Lunde, Per Kristian Frisk, Michael Aronsen, Jan Magnus Norden, Einar Sjaastad Cataliotti, Alessandro Sjaastad, Ivar Sejersted, Ole M Edwards, Andrew Lines, Glenn Terje Louch, William Edward . Ryanodine receptor dispersion disrupts Ca2+ release in failing cardiac myocytes. eLIFE. 2018, 7 | |
dc.identifier.uri | http://hdl.handle.net/10852/67967 | |
dc.description.abstract | Reduced cardiac contractility during heart failure (HF) is linked to impaired Ca2+ release from Ryanodine Receptors (RyRs). We investigated whether this deficit can be traced to nanoscale RyR reorganization. Using super-resolution imaging, we observed dispersion of RyR clusters in cardiomyocytes from post-infarction HF rats, resulting in more numerous, smaller clusters. Functional groupings of RyR clusters which produce Ca2+ sparks (Ca2+ release units, CRUs) also became less solid. An increased fraction of small CRUs in HF was linked to augmented ‘silent’ Ca2+ leak, not visible as sparks. Larger multi-cluster CRUs common in HF also exhibited low fidelity spark generation. When successfully triggered, sparks in failing cells displayed slow kinetics as Ca2+ spread across dispersed CRUs. During the action potential, these slow sparks protracted and desynchronized the overall Ca2+ transient. Thus, nanoscale RyR reorganization during HF augments Ca2+ leak and slows Ca2+ release kinetics, leading to weakened contraction in this disease. | en_US |
dc.language | EN | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Ryanodine receptor dispersion disrupts Ca2+ release in failing cardiac myocytes | en_US |
dc.type | Journal article | en_US |
dc.creator.author | Kolstad, Terje R Selnes | |
dc.creator.author | Brink, Jonas van Den | |
dc.creator.author | Macquaide, Niall | |
dc.creator.author | Lunde, Per Kristian | |
dc.creator.author | Frisk, Michael | |
dc.creator.author | Aronsen, Jan Magnus | |
dc.creator.author | Norden, Einar Sjaastad | |
dc.creator.author | Cataliotti, Alessandro | |
dc.creator.author | Sjaastad, Ivar | |
dc.creator.author | Sejersted, Ole M | |
dc.creator.author | Edwards, Andrew | |
dc.creator.author | Lines, Glenn Terje | |
dc.creator.author | Louch, William Edward | |
cristin.unitcode | 185,53,15,10 | |
cristin.unitname | Institutt for eksperimentell medisinsk forskning | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 1647794 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=eLIFE&rft.volume=7&rft.spage=&rft.date=2018 | |
dc.identifier.jtitle | eLIFE | |
dc.identifier.volume | 7 | |
dc.identifier.doi | http://dx.doi.org/10.7554/eLife.39427 | |
dc.identifier.urn | URN:NBN:no-71131 | |
dc.type.document | Tidsskriftartikkel | en_US |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 2050-084X | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/67967/1/elife-39427-v2.pdf | |
dc.type.version | PublishedVersion | |
cristin.articleid | e39427 | |