dc.date.accessioned | 2019-04-03T13:31:58Z | |
dc.date.available | 2019-04-03T13:31:58Z | |
dc.date.created | 2018-11-05T10:38:02Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Abrahamsen, Greger Gjerstad, Vibeke Sundvold Mekonnen, Meseret Bogen, Bjarne Spurkland, Anne . Polarity of CD4+ T cells towards the antigen presenting cell is regulated by the Lck adapter TSAd. Scientific Reports. 2018, 8:13319, 1-13 | |
dc.identifier.uri | http://hdl.handle.net/10852/67515 | |
dc.description.abstract | Polarization of T cells towards the antigen presenting cell (APC) is critically important for appropriate activation and differentiation of the naïve T cell. Here we used imaging flow cytometry (IFC) and show that the activation induced Lck and Itk adapter T cell specific adapter protein (TSAd), encoded by SH2D2A, modulates polarization of T cells towards the APC. Upon exposure to APC presenting the cognate antigen Id, Sh2d2a−/− CD4+ T cells expressing Id-specific transgenic T cell receptor (TCR), displayed impaired polarization of F-actin and TCR to the immunological synapse (IS). Sh2d2a−/− T-cells that did polarize F-actin and TCR still displayed impaired polarization of PKCξ, PAR3 and the microtubule-organizing center (MTOC). In vitro differentiation of activated Sh2d2a−/− T cells was skewed towards an effector memory (Tem) rather than a central memory (Tcm) phenotype. A similar trend was observed for Id-specific TCR Sh2d2a−/− T cells stimulated with APC and cognate antigen. Taken together our data suggest that TSAd modulates differentiation of experienced T cells possibly through polarization of CD4+ T cells towards the APC. | en_US |
dc.language | EN | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Polarity of CD4+ T cells towards the antigen presenting cell is regulated by the Lck adapter TSAd | en_US |
dc.type | Journal article | en_US |
dc.creator.author | Abrahamsen, Greger | |
dc.creator.author | Gjerstad, Vibeke Sundvold | |
dc.creator.author | Mekonnen, Meseret | |
dc.creator.author | Bogen, Bjarne | |
dc.creator.author | Spurkland, Anne | |
cristin.unitcode | 185,51,12,38 | |
cristin.unitname | Molekylær immunologi | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 1626956 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Scientific Reports&rft.volume=8:13319&rft.spage=1&rft.date=2018 | |
dc.identifier.jtitle | Scientific Reports | |
dc.identifier.volume | 8:13319 | |
dc.identifier.startpage | 1 | |
dc.identifier.endpage | 13 | |
dc.identifier.doi | http://dx.doi.org/10.1038/s41598-018-31510-6 | |
dc.identifier.urn | URN:NBN:no-70688 | |
dc.type.document | Tidsskriftartikkel | en_US |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 2045-2322 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/67515/1/Abrahamsen_Sci%2BReports_Cristin-post%2B1626956.pdf | |
dc.type.version | PublishedVersion | |
dc.relation.project | KF/163359 | |
dc.relation.project | KF/163361 | |
dc.relation.project | NFR/196386 | |