dc.date.accessioned | 2019-03-21T13:26:33Z | |
dc.date.available | 2019-03-21T13:26:33Z | |
dc.date.created | 2018-07-12T14:44:38Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Gopalakrishnan Saroja, Seethapathy Tadesse, Margey Santhosh Kumar, JU Gunaga, Srikanth V. Vasudeva, Ramesh Malterud, Karl Egil Ramanan, Uma Shaanker de Boer, Hugo Gudasalamani, Ravikanth Wangensteen, Helle . Authentication of Garcinia fruits and food supplements using DNA barcoding and NMR spectroscopy. Scientific Reports. 2018, 8(10561) | |
dc.identifier.uri | http://hdl.handle.net/10852/67357 | |
dc.description.abstract | Garcinia L. (Clusiaceae) fruits are a rich source of (−)-hydroxycitric acid, and this has gained considerable attention as an anti-obesity agent and a popular weight loss food supplement. In this study, we assessed adulteration of morphologically similar samples of Garcinia using DNA barcoding, and used NMR to quantify the content of (−)-hydroxycitric acid and (−)-hydroxycitric acid lactone in raw herbal drugs and Garcinia food supplements. DNA barcoding revealed that mostly G. gummi-gutta (previously known as G. cambogia) and G. indica were traded in Indian herbal markets, and there was no adulteration. The content of (−)-hydroxycitric acid and (−)-hydroxycitric acid lactone in the two species varied from 1.7% to 16.3%, and 3.5% to 20.7% respectively. Analysis of ten Garcinia food supplements revealed a large variation in the content of (−)-hydroxycitric acid, from 29 mg (4.6%) to 289 mg (50.6%) content per capsule or tablet. Only one product contained quantifiable amounts of (−)-hydroxycitric acid lactone. Furthermore the study demonstrates that DNA barcoding and NMR could be effectively used as a regulatory tool to authenticate Garcinia fruit rinds and food supplements. | en_US |
dc.language | EN | |
dc.relation.ispartof | Seethapathy, G.S. (2019) Ethnobotany, phytochemistry and DNA metabarcoding studies on Indian traditional medicine. Doctoral thesis. http://hdl.handle.net/10852/68510 | |
dc.relation.uri | http://hdl.handle.net/10852/68510 | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Authentication of Garcinia fruits and food supplements using DNA barcoding and NMR spectroscopy | en_US |
dc.type | Journal article | en_US |
dc.creator.author | Gopalakrishnan Saroja, Seethapathy | |
dc.creator.author | Tadesse, Margey | |
dc.creator.author | Santhosh Kumar, JU | |
dc.creator.author | Gunaga, Srikanth V. | |
dc.creator.author | Vasudeva, Ramesh | |
dc.creator.author | Malterud, Karl Egil | |
dc.creator.author | Ramanan, Uma Shaanker | |
dc.creator.author | de Boer, Hugo | |
dc.creator.author | Gudasalamani, Ravikanth | |
dc.creator.author | Wangensteen, Helle | |
cristin.unitcode | 185,15,23,20 | |
cristin.unitname | Farmasøytisk kjemi | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 1596962 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Scientific Reports&rft.volume=8&rft.spage=&rft.date=2018 | |
dc.identifier.jtitle | Scientific Reports | |
dc.identifier.volume | 8 | |
dc.identifier.issue | 10561 | |
dc.identifier.pagecount | 12 | |
dc.identifier.doi | http://dx.doi.org/10.1038/s41598-018-28635-z | |
dc.identifier.urn | URN:NBN:no-70525 | |
dc.type.document | Tidsskriftartikkel | en_US |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 2045-2322 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/67357/2/Scientific%2BReports%2B2018%25288%252910561.pdf | |
dc.type.version | PublishedVersion | |