dc.date.accessioned | 2018-09-12T09:18:21Z | |
dc.date.available | 2018-09-12T09:18:21Z | |
dc.date.created | 2017-06-09T20:53:14Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Poothong, Somrutai Lundanes, Elsa Thomsen, Cathrine Haug, Line Småstuen . High throughput online solid phase extraction-ultra high performance liquid chromatography-tandem mass spectrometry method for polyfluoroalkyl phosphate esters, perfluoroalkyl phosphonates, and other perfluoroalkyl substances in human serum, plasma, and whole blood. Analytica Chimica Acta. 2017, 957, 10-19 | |
dc.identifier.uri | http://hdl.handle.net/10852/64644 | |
dc.description.abstract | A rapid, sensitive and reliable method was developed for the determination of a broad range of poly- and perfluoroalkyl substances (PFASs) in various blood matrices (serum, plasma, and whole blood), and uses only 50 μL of sample material. The method consists of a rapid protein precipitation by methanol followed by high throughput online solid phase extraction (SPE), ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS), and negative electrospray ionization detection. The method was developed for simultaneous determination of twenty-five PFASs, including polyfluoroalkyl phosphate esters (PAPs; 6:2, 8:2, 6:2/6:2, and 8:2/8:2), perfluoroalkyl phosphonates (PFPAs; C6, C8, and C10), perfluoroalkyl sulfonates (PFSAs; C4, C6, C7, C8, and C10), perfluoroalkyl carboxylates (PFCAs; C5C14), and perfluoroalkyl sulfonamides (FOSAs; C8, N-methyl, and N-ethyl). High linearity of matrix-matched calibration standards (correlation coefficients, R = 0.99–0.999) were obtained in the range of 0.006–45 ng mL−1 blood. Excellent sensitivity was achieved with method detection limits (MDLs) between 0.0018 and 0.09 ng mL−1, depending on the compound and matrix. The method was validated for serum, plasma, and whole blood (n = 5 + 5) at six levels in the range 0.0180–30 ng mL−1. The accuracy (n = 5) was on average 102± 12%. The intermediate precision (n = 10) ranged from 2 to 40% with an average between-batch of analyses difference of 10± 10%. Two human serum samples from a former interlaboratory comparison were analyzed and the differences between the applied method and the consensus values were below ≤22% (n = 5). The method was also successfully applied to samples of human plasma and whole blood with coefficients of variation in the range 0.8–15.2% (n = 5). | en_US |
dc.language | EN | |
dc.publisher | Elsevier Science | |
dc.relation.ispartof | Poothong, Somrutai (2018) Poly- and perfluoroalkyl substances (PFASs): from external exposure to human blood. Doctoral thesis. http://hdl.handle.net/10852/65978 | |
dc.relation.uri | http://hdl.handle.net/10852/65978 | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.title | High throughput online solid phase extraction-ultra high performance liquid chromatography-tandem mass spectrometry method for polyfluoroalkyl phosphate esters, perfluoroalkyl phosphonates, and other perfluoroalkyl substances in human serum, plasma, and whole blood | en_US |
dc.type | Journal article | en_US |
dc.creator.author | Poothong, Somrutai | |
dc.creator.author | Lundanes, Elsa | |
dc.creator.author | Thomsen, Cathrine | |
dc.creator.author | Haug, Line Småstuen | |
cristin.unitcode | 185,15,12,0 | |
cristin.unitname | Kjemisk institutt | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 1475062 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Analytica Chimica Acta&rft.volume=957&rft.spage=10&rft.date=2017 | |
dc.identifier.jtitle | Analytica Chimica Acta | |
dc.identifier.volume | 957 | |
dc.identifier.startpage | 10 | |
dc.identifier.endpage | 19 | |
dc.identifier.doi | http://dx.doi.org/10.1016/j.aca.2016.12.043 | |
dc.identifier.urn | URN:NBN:no-67182 | |
dc.type.document | Tidsskriftartikkel | en_US |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 0003-2670 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/64644/2/1-s2.0-S0003267016315185-main.pdf | |
dc.type.version | PublishedVersion | |
dc.relation.project | EC/FP7/316665 | |