dc.date.accessioned | 2018-08-17T07:12:54Z | |
dc.date.available | 2018-08-17T07:12:54Z | |
dc.date.created | 2018-07-03T09:12:49Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Grevys, Algirdas Nilsen, Jeannette Sand, Kine Marita Knudsen Daba, Muluneh Bekele Øynebråten, Inger Bern, Malin C. McAdam, Martin Berner Foss, Stian Schlothauer, Tilman Michaelsen, Terje Einar Christianson, Gregory J. Roopenian, Derry C. Blumberg, Richard S. Sandlie, Inger Andersen, Jan Terje . A human endothelial cell-based recycling assay for screening of FcRn targeted molecules. Nature Communications. 2018, 9 | |
dc.identifier.uri | http://hdl.handle.net/10852/63024 | |
dc.description.abstract | Albumin and IgG have remarkably long serum half-lives due to pH-dependent FcRn-mediated cellular recycling that rescues both ligands from intracellular degradation. Furthermore, increase in half-lives of IgG and albumin-based therapeutics has the potential to improve their efficacies, but there is a great need for robust methods for screening of relative FcRn-dependent recycling ability. Here, we report on a novel human endothelial cell-based recycling assay (HERA) that can be used for such pre-clinical screening. In HERA, rescue from degradation depends on FcRn, and engineered ligands are recycled in a manner that correlates with their half-lives in human FcRn transgenic mice. Thus, HERA is a novel cellular assay that can be used to predict how FcRn-binding proteins are rescued from intracellular degradation. | en_US |
dc.language | EN | |
dc.language.iso | en | en_US |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | A human endothelial cell-based recycling assay for screening of FcRn targeted molecules | en_US |
dc.title.alternative | ENEngelskEnglishA human endothelial cell-based recycling assay for screening of FcRn targeted molecules | |
dc.type | Journal article | en_US |
dc.creator.author | Grevys, Algirdas | |
dc.creator.author | Nilsen, Jeannette | |
dc.creator.author | Sand, Kine Marita Knudsen | |
dc.creator.author | Daba, Muluneh Bekele | |
dc.creator.author | Øynebråten, Inger | |
dc.creator.author | Bern, Malin C. | |
dc.creator.author | McAdam, Martin Berner | |
dc.creator.author | Foss, Stian | |
dc.creator.author | Schlothauer, Tilman | |
dc.creator.author | Michaelsen, Terje Einar | |
dc.creator.author | Christianson, Gregory J. | |
dc.creator.author | Roopenian, Derry C. | |
dc.creator.author | Blumberg, Richard S. | |
dc.creator.author | Sandlie, Inger | |
dc.creator.author | Andersen, Jan Terje | |
cristin.unitcode | 185,53,2,11 | |
cristin.unitname | Senter for immunregulering | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 2 | |
dc.identifier.cristin | 1595337 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Nature Communications&rft.volume=9&rft.spage=&rft.date=2018 | |
dc.identifier.jtitle | Nature Communications | |
dc.identifier.volume | 9 | |
dc.identifier.pagecount | 14 | |
dc.identifier.doi | http://dx.doi.org/10.1038/s41467-018-03061-x | |
dc.identifier.urn | URN:NBN:no-65589 | |
dc.type.document | Tidsskriftartikkel | en_US |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 2041-1723 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/63024/2/Grevys_Andersen_Nat%2BCommun_Cristin-post%2B1595337.pdf | |
dc.type.version | PublishedVersion | |
cristin.articleid | 621 | |
dc.relation.project | NFR/179573 | |
dc.relation.project | NFR/230526 | |
dc.relation.project | NFR/143822 | |