dc.date.accessioned | 2018-08-17T06:51:57Z | |
dc.date.available | 2018-08-17T06:51:57Z | |
dc.date.created | 2018-06-20T13:09:40Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Thomas, Anub Mathew Schjalm, Camilla Nilsson, Per Lindenskov, Paal Helge H. Rørtveit, Runa Solberg, Rønnaug Saugstad, Ola Didrik Berglund, Magnus M. Strömberg, Patrik Lau, Corinna Espevik, Terje Jansen, Johan Høgset Castellheim, Albert Mollnes, Tom Eirik Barratt-Due, Andreas . Combined Inhibition of C5 and CD14 Attenuates Systemic Inflammation in a Piglet Model of Meconium Aspiration Syndrome. Neonatology. 2018, 113(4), 322-330 | |
dc.identifier.uri | http://hdl.handle.net/10852/63022 | |
dc.description.abstract | Background: Meconium aspiration syndrome (MAS) is a severe lung condition affecting newborns and it can lead to a systemic inflammatory response. We previously documented complement activation and cytokine release in a piglet MAS model. Additionally, we showed ex vivo that meconium-induced inflammation was dependent on complement and Toll-like receptors. Objectives: To assess the efficacy of the combined inhibition of complement (C5) and CD14 on systemic inflammation induced in a forceful piglet MAS model. Methods: Thirty piglets were randomly allocated to a treatment group receiving the C5-inhibitor SOBI002 and anti-CD14 (n = 15) and a nontreated control group (n = 15). MAS was induced by intratracheal meconium instillation, and the piglets were observed for 5 h. Complement, cytokines, and myeloperoxidase (MPO) were measured by ELISA. Results: SOBI002 ablated C5 activity and the formation of the terminal complement complex in vivo. The combined inhibition attenuated the inflammasome cytokines IL-1β and IL-6 by 60 (p = 0.029) and 44% (p = 0.01), respectively, and also MPO activity in the bronchoalveolar fluid by 42% (p = 0.017). Ex vivo experiments in human blood revealed that the combined regimen attenuated meconium-induced MPO release by 64% (p = 0.008), but there was only a negligible effect with single inhibition, indicating a synergic cross-talk between the key molecules C5 and CD14. Conclusion: Combined inhibition of C5 and CD14 attenuates meconium-induced inflammation in vivo and this could become a future therapeutic regimen for MAS. | en_US |
dc.language | EN | |
dc.language.iso | en | en_US |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.title | Combined Inhibition of C5 and CD14 Attenuates Systemic Inflammation in a Piglet Model of Meconium Aspiration Syndrome | en_US |
dc.title.alternative | ENEngelskEnglishCombined Inhibition of C5 and CD14 Attenuates Systemic Inflammation in a Piglet Model of Meconium Aspiration Syndrome | |
dc.type | Journal article | en_US |
dc.creator.author | Thomas, Anub Mathew | |
dc.creator.author | Schjalm, Camilla | |
dc.creator.author | Nilsson, Per | |
dc.creator.author | Lindenskov, Paal Helge H. | |
dc.creator.author | Rørtveit, Runa | |
dc.creator.author | Solberg, Rønnaug | |
dc.creator.author | Saugstad, Ola Didrik | |
dc.creator.author | Berglund, Magnus M. | |
dc.creator.author | Strömberg, Patrik | |
dc.creator.author | Lau, Corinna | |
dc.creator.author | Espevik, Terje | |
dc.creator.author | Jansen, Johan Høgset | |
dc.creator.author | Castellheim, Albert | |
dc.creator.author | Mollnes, Tom Eirik | |
dc.creator.author | Barratt-Due, Andreas | |
cristin.unitcode | 185,53,18,71 | |
cristin.unitname | K.G. Jebsen Senter for betennelsesforskning - part UiO | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 1592619 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Neonatology&rft.volume=113&rft.spage=322&rft.date=2018 | |
dc.identifier.jtitle | Neonatology | |
dc.identifier.volume | 113 | |
dc.identifier.issue | 4 | |
dc.identifier.startpage | 322 | |
dc.identifier.endpage | 330 | |
dc.identifier.doi | http://dx.doi.org/10.1159/000486542 | |
dc.identifier.urn | URN:NBN:no-65586 | |
dc.type.document | Tidsskriftartikkel | en_US |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 1661-7800 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/63022/1/Thomas_et_al.pdf | |
dc.type.version | PublishedVersion | |