dc.date.accessioned | 2018-06-12T10:59:31Z | |
dc.date.available | 2018-06-12T10:59:31Z | |
dc.date.created | 2018-01-24T15:34:32Z | |
dc.date.issued | 1995 | |
dc.identifier.citation | Jacobsen, Sten Eirik W. Okkenhaug, Cecilie Myklebust, June Veiby, Ole-Petter Lyman, S. . The FLT3 ligand potently and directly stimulates the growth and expansion of primitive murine bone marrow progenitor cells in vitro: synergistic interactions with interleukin (IL) 11, IL-12, and other hematopoietic growth factors. Journal of Experimental Medicine. 1995, 181(4), 1357-1363 | |
dc.identifier.uri | http://hdl.handle.net/10852/61856 | |
dc.description.abstract | The recently cloned murine flt3 ligand (FL) was studied for its ability to stimulate the growth of primitive (Lin-Sca-1+) and more committed (Lin-Sca-1-) murine bone marrow progenitor cells, alone and in combination with other hematopoietic growth factors (HGFs). Whereas FL was a weak proliferative stimulator alone, it potently synergized with a number of other HGFs, including all four colony-stimulating factor (CSF), interleukin (IL) 6, IL-11, IL-12, and stem cell factor (SCF), to promote the colony formation of Lin-Sca-1+, but not Lin-Sca-1- or erythroid progenitor cells. The synergistic activity of FL was concentration dependent, with maximum stimulation occurring at 250 ng/ml, and was observed when cells were plated at a concentration of one cell per culture, suggesting that its effects are directly mediated. 2 wk of treatment with FL in combination with IL-3 or SCF resulted in the production of a high proportion of mature myeloid cells (granulocytes and macrophages), whereas the combination of FL with G- CSF, IL-11, or IL-12 resulted predominantly in the formation of cells with an immature blast cell appearance. Accordingly, FL in combination with G-CSF or IL-11 expanded the number of progenitors more than 40- fold after 2 wk incubation. Thus, FL emerges as a potent synergistic HGF, that in combination with numerous other HGFs, can directly stimulate the proliferation, myeloid differentiation, and expansion of primitive hematopoietic progenitor cells. | en_US |
dc.language | EN | |
dc.title | The FLT3 ligand potently and directly stimulates the growth and expansion of primitive murine bone marrow progenitor cells in vitro: synergistic interactions with interleukin (IL) 11, IL-12, and other hematopoietic growth factors | en_US |
dc.type | Journal article | en_US |
dc.creator.author | Jacobsen, Sten Eirik W. | |
dc.creator.author | Okkenhaug, Cecilie | |
dc.creator.author | Myklebust, June | |
dc.creator.author | Veiby, Ole-Petter | |
dc.creator.author | Lyman, S. | |
cristin.unitcode | 185,53,49,12 | |
cristin.unitname | Institutt for kreftforskning | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 2 | |
dc.identifier.cristin | 1551182 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal of Experimental Medicine&rft.volume=181&rft.spage=1357&rft.date=1995 | |
dc.identifier.jtitle | Journal of Experimental Medicine | |
dc.identifier.volume | 181 | |
dc.identifier.issue | 4 | |
dc.identifier.startpage | 1357 | |
dc.identifier.endpage | 1363 | |
dc.identifier.urn | URN:NBN:no-64461 | |
dc.type.document | Tidsskriftartikkel | en_US |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 0022-1007 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/61856/1/Jacobsen_J%2BExp%2BMed%2B1995.pdf | |
dc.type.version | PublishedVersion | |