dc.date.accessioned | 2018-06-05T12:27:23Z | |
dc.date.available | 2018-06-05T12:27:23Z | |
dc.date.created | 2016-08-15T16:25:41Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Dahal-Koirala, Shiva Risnes, Louise Fremgaard Christophersen, Asbjørn Sarna, Vikas Kumar Lundin, Knut Erik Aslaksen Sollid, Ludvig Magne Qiao, Shuo Wang . TCR sequencing of single cells reactive to DQ2.5-glia-α2 and DQ2.5-glia-ω2 reveals clonal expansion and epitope-specific V-gene usage. Mucosal Immunology. 2016, 9(3), 587-596 | |
dc.identifier.uri | http://hdl.handle.net/10852/61796 | |
dc.description.abstract | CD4+ T cells recognizing dietary gluten epitopes in the context of disease-associated human leukocyte antigen (HLA)-DQ2 or HLA-DQ8 molecules are the key players in celiac disease pathogenesis. Here, we conducted a large-scale single-cell paired T-cell receptor (TCR) sequencing study to characterize the TCR repertoire for two homologous immunodominant gluten epitopes, DQ2.5-glia-α2 and DQ2.5-glia-ω2, in blood of celiac disease patients after oral gluten challenge. Despite sequence similarity of the epitopes, the TCR repertoires are unique but shared several overall features. We demonstrate that clonally expanded T cells dominate the T-cell responses to both epitopes. Moreover, we find V-gene bias of TRAV26, TRAV4, and TRBV7 in DQ2.5-glia-α2 reactive TCRs, while DQ2.5-glia-ω2 TCRs displayed significant bias toward TRAV4 and TRBV4. The knowledge that antigen-specific TCR repertoire in chronic inflammatory diseases tends to be dominated by a few expanded clones that use the same TCR V-gene segments across patients is important information for HLA-associated diseases where the antigen is unknown. | en_US |
dc.language | EN | |
dc.title | TCR sequencing of single cells reactive to DQ2.5-glia-α2 and DQ2.5-glia-ω2 reveals clonal expansion and epitope-specific V-gene usage | en_US |
dc.type | Journal article | en_US |
dc.creator.author | Dahal-Koirala, Shiva | |
dc.creator.author | Risnes, Louise Fremgaard | |
dc.creator.author | Christophersen, Asbjørn | |
dc.creator.author | Sarna, Vikas Kumar | |
dc.creator.author | Lundin, Knut Erik Aslaksen | |
dc.creator.author | Sollid, Ludvig Magne | |
dc.creator.author | Qiao, Shuo Wang | |
cristin.unitcode | 185,53,18,12 | |
cristin.unitname | Avdeling for immunologi og transfusjonsmedisin | |
cristin.ispublished | true | |
cristin.fulltext | preprint | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 1372949 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Mucosal Immunology&rft.volume=9&rft.spage=587&rft.date=2016 | |
dc.identifier.jtitle | Mucosal Immunology | |
dc.identifier.volume | 9 | |
dc.identifier.issue | 3 | |
dc.identifier.startpage | 587 | |
dc.identifier.endpage | 596 | |
dc.identifier.doi | http://dx.doi.org/10.1038/mi.2015.147 | |
dc.identifier.urn | URN:NBN:no-64403 | |
dc.type.document | Tidsskriftartikkel | en_US |
dc.source.issn | 1935-3456 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/61796/2/a2w2%2Barticle_SDK_%2B160915.pdf | |
dc.type.version | SubmittedVersion | |
dc.relation.project | NFR/179573 | |