dc.date.accessioned | 2018-03-21T14:44:33Z | |
dc.date.available | 2018-03-21T14:44:33Z | |
dc.date.created | 2017-01-18T09:41:22Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Manna, Dipankar Pust, Sascha Torgersen, Maria Lyngaas Cordara, Gabriele Kunzler, Markus Krengel, Ute Sandvig, Kirsten . Polyporus squamosus lectin 1a (PSL1a) exhibits cytotoxicity in mammalian cells by disruption of focal adhesions, inhibition of protein synthesis and induction of apoptosis. PLoS ONE. 2017, 12(1) | |
dc.identifier.uri | http://hdl.handle.net/10852/61213 | |
dc.description.abstract | PSL1a is a lectin from the mushroom Polyporus squamosus that binds to sialylated glycans and glycoconjugates with high specificity and selectivity. In addition to its N-terminal carbohydrate-binding domain, PSL1a possesses a Ca2+-dependent proteolytic activity in the C-terminal domain. In the present study, we demonstrate that PSL1a has cytotoxic effects on mammalian cancer cells, and we show that the cytotoxicity is dependent on the cysteine protease activity. PSL1a treatment leads to cell rounding and detachment from the substratum, concomitant with disruption of vinculin complexes in focal adhesions. We also demonstrate that PSL1a inhibits protein synthesis and induces apoptosis in HeLa cells, in a time- and concentration-dependent manner. | en_US |
dc.language | EN | |
dc.publisher | Public Library of Science (PLoS) | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Polyporus squamosus lectin 1a (PSL1a) exhibits cytotoxicity in mammalian cells by disruption of focal adhesions, inhibition of protein synthesis and induction of apoptosis | en_US |
dc.type | Journal article | en_US |
dc.creator.author | Manna, Dipankar | |
dc.creator.author | Pust, Sascha | |
dc.creator.author | Torgersen, Maria Lyngaas | |
dc.creator.author | Cordara, Gabriele | |
dc.creator.author | Kunzler, Markus | |
dc.creator.author | Krengel, Ute | |
dc.creator.author | Sandvig, Kirsten | |
cristin.unitcode | 185,15,12,0 | |
cristin.unitname | Kjemisk institutt | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 1430381 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=PLoS ONE&rft.volume=12&rft.spage=&rft.date=2017 | |
dc.identifier.jtitle | PLoS ONE | |
dc.identifier.volume | 12 | |
dc.identifier.issue | 1 | |
dc.identifier.doi | http://dx.doi.org/10.1371/journal.pone.0170716 | |
dc.identifier.urn | URN:NBN:no-63832 | |
dc.type.document | Tidsskriftartikkel | en_US |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 1932-6203 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/61213/2/PloS%2BOne%2B2017%2BManna.pdf | |
dc.type.version | PublishedVersion | |
dc.relation.project | NFR/179571 | |