dc.date.accessioned | 2018-03-13T14:38:50Z | |
dc.date.available | 2019-03-01T23:46:40Z | |
dc.date.created | 2018-01-15T17:00:56Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Dermauw, Veronique Carabin, Helene Cisse, A. Millogo, A Tarnagda, Z Ganaba, Rasmane Noh, John Handali, S Breen, K Richter, V Cissé, R Preux, PM Boncoeur-Martel, MP Winkler, Andrea Sylvia Van Hul, A Dorny, P. Gabriël, S . Evaluating the Recombinant T24H Enzyme-Linked Immunoelectrotransfer Blot Assay for the Diagnosis of Neurocysticercosis in a Panel of Samples from a Large Community-Based Randomized Control Trial in 60 Villages in Burkina Faso.. American Journal of Tropical Medicine and Hygiene. 2017 | |
dc.identifier.uri | http://hdl.handle.net/10852/60947 | |
dc.description.abstract | Current guidelines for the diagnosis of neurocysticercosis (NCC) recommend the use of the lentil lectin-bound glycoprotein enzyme-linked immunoelectrotransfer blot assay (LLGP-EITB) as the reference standard for serological testing. In response to the drawbacks involved with the use of the LLGP-EITB, a recombinant T24H antigen (rT24H) EITB assay was developed, with promising results. However, the test has yet to be evaluated among individuals from sub-Saharan Africa (SSA). The aim of the present study was to investigate the performance of the rT24H EITB assay for the detection of NCC cases in a panel of serum samples (N = 366, of which 173 patients presented with epileptic seizures and/or severe chronic headaches, and 193 matched manifestation-free participants) collected as part of a large community-based trial in Burkina Faso. A perfect agreement between the rT24H EITB and the native gp24 (and its homodimer, gp42) LLGP-EITB was found (kappa value of 1.0). Furthermore, among patients with the neurological manifestations of interest who underwent a computed tomography scan, the rT24H EITB and native antigen LLGP-EITB had a comparable ability to correctly identify NCC cases with multiple viable (rT24H: sensitivity: 80.0%), single viable (66.7%), and calcified/degenerating cysts only (25.0%), albeit for multiple viable and calcified cysts, the rT24H estimated sensitivity seemed lower, but more uncertain, than previously reported. The rT24H EITB specificity was high (98.2%) and in line with previous studies. This study confirms the value of the recombinant rT24H EITB as an alternative to the native antigen LLGP-EITB for the diagnosis of NCC in a SSA community setting. | |
dc.language | EN | |
dc.language.iso | en | en_US |
dc.publisher | HighWire Press | |
dc.title | Evaluating the Recombinant T24H Enzyme-Linked Immunoelectrotransfer Blot Assay for the Diagnosis of Neurocysticercosis in a Panel of Samples from a Large Community-Based Randomized Control Trial in 60 Villages in Burkina Faso. | en_US |
dc.type | Journal article | en_US |
dc.creator.author | Dermauw, Veronique | |
dc.creator.author | Carabin, Helene | |
dc.creator.author | Cisse, A. | |
dc.creator.author | Millogo, A | |
dc.creator.author | Tarnagda, Z | |
dc.creator.author | Ganaba, Rasmane | |
dc.creator.author | Noh, John | |
dc.creator.author | Handali, S | |
dc.creator.author | Breen, K | |
dc.creator.author | Richter, V | |
dc.creator.author | Cissé, R | |
dc.creator.author | Preux, PM | |
dc.creator.author | Boncoeur-Martel, MP | |
dc.creator.author | Winkler, Andrea Sylvia | |
dc.creator.author | Van Hul, A | |
dc.creator.author | Dorny, P. | |
dc.creator.author | Gabriël, S | |
cristin.unitcode | 185,52,14,0 | |
cristin.unitname | Avdeling for samfunnsmedisin og global helse | |
cristin.ispublished | true | |
cristin.fulltext | postprint | |
cristin.fulltext | postprint | |
cristin.fulltext | postprint | |
cristin.fulltext | postprint | |
cristin.fulltext | postprint | |
cristin.fulltext | postprint | |
cristin.fulltext | postprint | |
cristin.fulltext | postprint | |
cristin.fulltext | postprint | |
cristin.fulltext | postprint | |
cristin.fulltext | postprint | |
cristin.fulltext | postprint | |
cristin.fulltext | postprint | |
cristin.fulltext | original | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 1543391 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=American Journal of Tropical Medicine and Hygiene&rft.volume=&rft.spage=&rft.date=2017 | |
dc.identifier.jtitle | American Journal of Tropical Medicine and Hygiene | |
dc.identifier.volume | 98 | |
dc.identifier.issue | 2 | |
dc.identifier.startpage | 565 | |
dc.identifier.endpage | 569 | |
dc.identifier.doi | http://dx.doi.org/10.4269/ajtmh.17-0541 | |
dc.identifier.urn | URN:NBN:no-63577 | |
dc.type.document | Tidsskriftartikkel | en_US |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 0002-9637 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/60947/15/tpmd170541.pdf | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/60947/16/suppl-tpmd170541-tpmd170541.SD1.pdf | |
dc.type.version | PublishedVersion | |