dc.date.accessioned | 2018-02-14T17:43:33Z | |
dc.date.available | 2018-02-14T17:43:33Z | |
dc.date.created | 2013-02-08T10:34:37Z | |
dc.date.issued | 2012 | |
dc.identifier.citation | Nordstrand, Line Mari Furu, Kari Paulsen, Jonas Rognes, Torbjørn Klungland, Arne . Alkbh1 and Tzfp repress a non-repeat piRNA cluster in pachytene spermatocytes. Nucleic Acids Research. 2012, 40(21), 10950-10963 | |
dc.identifier.uri | http://hdl.handle.net/10852/60104 | |
dc.description.abstract | Piwi proteins and Piwi-interacting small RNAs (piRNAs) have known functions in transposon silencing in the male germline of fetal and newborn mice. Both are also present in adult testes; however, their function here remains a mystery. Here, we confirm that most piRNAs in meiotic spermatocytes originate from clusters in non-repeat intergenic regions of DNA. The regulation of these piRNA clusters, including the processing of the precursor transcripts into individual piRNAs, is accomplished through mostly unknown processes. We present a possible regulatory mechanism for one such cluster, named cluster 1082B, located on chromosome 7 in the mouse genome. The 1082B precursor transcript and its 788 unique piRNAs are repressed by the Alkbh1 dioxygenase and the testis-specific transcription repressor Tzfp. We observe a remarkable >1000-fold upregulation of individual piRNAs in pachytene spermatocytes isolated from Alkbh1- and Tzfp -deficient murine testes. Repression of cluster 1082B is further supported by the identification of a 10-bp Tzfp recognition sequence contained within the precursor transcript. Downregulation of LINE1 and IAP transcripts in the Alkbh1- and Tzfp -deficient mice leads us to propose a potential role for the 1082B-encoded piRNAs in transposon control. | en_US |
dc.language | EN | |
dc.language.iso | en | en_US |
dc.publisher | Oxford University Press | |
dc.rights | Attribution-NonCommercial 3.0 Unported | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc/3.0/ | |
dc.title | Alkbh1 and Tzfp repress a non-repeat piRNA cluster in pachytene spermatocytes | en_US |
dc.type | Journal article | en_US |
dc.creator.author | Nordstrand, Line Mari | |
dc.creator.author | Furu, Kari | |
dc.creator.author | Paulsen, Jonas | |
dc.creator.author | Rognes, Torbjørn | |
dc.creator.author | Klungland, Arne | |
cristin.unitcode | 185,15,5,35 | |
cristin.unitname | Forskningsgruppen for biomedisinsk informatikk | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 2 | |
dc.identifier.cristin | 1008112 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Nucleic Acids Research&rft.volume=40&rft.spage=10950&rft.date=2012 | |
dc.identifier.jtitle | Nucleic Acids Research | |
dc.identifier.volume | 40 | |
dc.identifier.issue | 21 | |
dc.identifier.startpage | 10950 | |
dc.identifier.endpage | 10963 | |
dc.identifier.doi | http://dx.doi.org/10.1093/nar/gks839 | |
dc.identifier.urn | URN:NBN:no-62773 | |
dc.type.document | Tidsskriftartikkel | en_US |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 0305-1048 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/60104/2/2012%2BNordstrand%2BAlkbh1%2Bpirna%2BNAR.pdf | |
dc.type.version | PublishedVersion | |