dc.date.accessioned | 2017-08-08T09:23:57Z | |
dc.date.available | 2017-08-08T09:23:57Z | |
dc.date.created | 2015-02-10T19:38:45Z | |
dc.date.issued | 2014 | |
dc.identifier.citation | de Been, Mark Lanza, Val F. de Toro, Maria Scharringa, Jelle Dohmen, Wietske Du, Yu Hu, Juan Lei, Ying Li, Ning Tooming-Klunderud, Ave Heederik, Dick J. J. Fluit, Ad C Bonten, Marc J M Willems, Rob J.L. de la Cruz, Fernando van Schaik, Willem . Dissemination of Cephalosporin Resistance Genes between Escherichia coli Strains from Farm Animals and Humans by Specific Plasmid Lineages. PLoS Genetics. 2014, 10(12) | |
dc.identifier.uri | http://hdl.handle.net/10852/56838 | |
dc.description.abstract | Third-generation cephalosporins are a class of β-lactam antibiotics that are often used for the treatment of human infections caused by Gram-negative bacteria, especially Escherichia coli. Worryingly, the incidence of human infections caused by third-generation cephalosporin-resistant E. coli is increasing worldwide. Recent studies have suggested that these E. coli strains, and their antibiotic resistance genes, can spread from food-producing animals, via the food-chain, to humans. However, these studies used traditional typing methods, which may not have provided sufficient resolution to reliably assess the relatedness of these strains. We therefore used whole-genome sequencing (WGS) to study the relatedness of cephalosporin-resistant E. coli from humans, chicken meat, poultry and pigs. One strain collection included pairs of human and poultry-associated strains that had previously been considered to be identical based on Multi-Locus Sequence Typing, plasmid typing and antibiotic resistance gene sequencing. The second collection included isolates from farmers and their pigs. WGS analysis revealed considerable heterogeneity between human and poultry-associated isolates. The most closely related pairs of strains from both sources carried 1263 Single-Nucleotide Polymorphisms (SNPs) per Mbp core genome. In contrast, epidemiologically linked strains from humans and pigs differed by only 1.8 SNPs per Mbp core genome. WGS-based plasmid reconstructions revealed three distinct plasmid lineages (IncI1- and IncK-type) that carried cephalosporin resistance genes of the Extended-Spectrum Beta-Lactamase (ESBL)- and AmpC-types. The plasmid backbones within each lineage were virtually identical and were shared by genetically unrelated human and animal isolates. Plasmid reconstructions from short-read sequencing data were validated by long-read DNA sequencing for two strains. Our findings failed to demonstrate evidence for recent clonal transmission of cephalosporin-resistant E. coli strains from poultry to humans, as has been suggested based on traditional, low-resolution typing methods. Instead, our data suggest that cephalosporin resistance genes are mainly disseminated in animals and humans via distinct plasmids. | en_US |
dc.language | EN | |
dc.publisher | Public Library of Science (PLoS) | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Dissemination of Cephalosporin Resistance Genes between Escherichia coli Strains from Farm Animals and Humans by Specific Plasmid Lineages | en_US |
dc.type | Journal article | en_US |
dc.creator.author | de Been, Mark | |
dc.creator.author | Lanza, Val F. | |
dc.creator.author | de Toro, Maria | |
dc.creator.author | Scharringa, Jelle | |
dc.creator.author | Dohmen, Wietske | |
dc.creator.author | Du, Yu | |
dc.creator.author | Hu, Juan | |
dc.creator.author | Lei, Ying | |
dc.creator.author | Li, Ning | |
dc.creator.author | Tooming-Klunderud, Ave | |
dc.creator.author | Heederik, Dick J. J. | |
dc.creator.author | Fluit, Ad C | |
dc.creator.author | Bonten, Marc J M | |
dc.creator.author | Willems, Rob J.L. | |
dc.creator.author | de la Cruz, Fernando | |
dc.creator.author | van Schaik, Willem | |
cristin.unitcode | 185,15,29,50 | |
cristin.unitname | Centre for Ecological and Evolutionary Synthesis | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.fulltext | original | |
cristin.qualitycode | 2 | |
dc.identifier.cristin | 1219848 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=PLoS Genetics&rft.volume=10&rft.spage=&rft.date=2014 | |
dc.identifier.jtitle | PLoS Genetics | |
dc.identifier.volume | 10 | |
dc.identifier.issue | 12 | |
dc.identifier.pagecount | 17 | |
dc.identifier.doi | http://dx.doi.org/10.1371/journal.pgen.1004776 | |
dc.identifier.urn | URN:NBN:no-59665 | |
dc.type.document | Tidsskriftartikkel | en_US |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 1553-7390 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/56838/1/journal.pgen.1004776.pdf | |
dc.type.version | PublishedVersion | |
cristin.articleid | e1004776 | |