dc.date.accessioned | 2017-08-04T10:07:53Z | |
dc.date.available | 2017-08-04T10:07:53Z | |
dc.date.created | 2012-11-07T14:52:03Z | |
dc.date.issued | 2012 | |
dc.identifier.citation | Brattelid, Trond Qvigstad, Eirik Moltzau, Lise Roman Bekkevold, Silje Veslemøy Sandnes, Dagny Lise Birkeland, Jon Arne Skomedal, Tor Osnes, Jan-Bjørn Sjaastad, Ivar Levy, Finn Olav . The Cardiac Ventricular 5-HT4 Receptor Is Functional in Late Foetal Development and Is Reactivated in Heart Failure. PLoS ONE. 2012, 7(9) | |
dc.identifier.uri | http://hdl.handle.net/10852/56768 | |
dc.description.abstract | A positive inotropic responsiveness to serotonin, mediated by 5-HT4 and 5-HT2A receptors, appears in the ventricle of rats with post-infarction congestive heart failure (HF) and pressure overload-induced hypertrophy. A hallmark of HF is a transition towards a foetal genotype which correlates with loss of cardiac functions. Thus, we wanted to investigate whether the foetal and neonatal cardiac ventricle displays serotonin responsiveness. Wistar rat hearts were collected day 3 and 1 before expected birth (days -3 and -1), as well as day 1, 3, 5 and 113 (age matched with Sham and HF) after birth. Hearts from post-infarction HF and sham-operated animals (Sham) were also collected. Heart tissue was examined for mRNA expression of 5-HT4, 5-HT2A and 5-HT2B serotonin receptors, 5-HT transporter, atrial natriuretic peptide (ANP) and myosin heavy chain (MHC)-α and MHC-β (real-time quantitative RT-PCR) as well as 5-HT-receptor-mediated increase in contractile function ex vivo (electrical field stimulation of ventricular strips from foetal and neonatal rats and left ventricular papillary muscle from adult rats in organ bath). Both 5-HT4 mRNA expression and functional responses were highest at day -3 and decreased gradually to day 5, with a further decrease to adult levels. In HF, receptor mRNA levels and functional responses reappeared, but to lower levels than in the foetal ventricle. The 5-HT2A and 5-HT2B receptor mRNA levels increased to a maximum immediately after birth, but of these, only the 5-HT2A receptor mediated a positive inotropic response. We suggest that the 5-HT4 receptor is a representative of a foetal cardiac gene program, functional in late foetal development and reactivated in heart failure. | en_US |
dc.language | EN | |
dc.publisher | Public Library of Science (PLoS) | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.rights.uri | Attribution 4.0 International | |
dc.title | The Cardiac Ventricular 5-HT4 Receptor Is Functional in Late Foetal Development and Is Reactivated in Heart Failure | en_US |
dc.type | Journal article | en_US |
dc.creator.author | Brattelid, Trond | |
dc.creator.author | Qvigstad, Eirik | |
dc.creator.author | Moltzau, Lise Roman | |
dc.creator.author | Bekkevold, Silje Veslemøy | |
dc.creator.author | Sandnes, Dagny Lise | |
dc.creator.author | Birkeland, Jon Arne | |
dc.creator.author | Skomedal, Tor | |
dc.creator.author | Osnes, Jan-Bjørn | |
dc.creator.author | Sjaastad, Ivar | |
dc.creator.author | Levy, Finn Olav | |
cristin.unitcode | 185,53,18,15 | |
cristin.unitname | Avdeling for farmakologi | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 960428 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=PLoS ONE&rft.volume=7&rft.spage=&rft.date=2012 | |
dc.identifier.jtitle | PLoS ONE | |
dc.identifier.volume | 7 | |
dc.identifier.issue | 9 | |
dc.identifier.doi | http://dx.doi.org/10.1371/journal.pone.0045489 | |
dc.identifier.urn | URN:NBN:no-59524 | |
dc.type.document | Tidsskriftartikkel | en_US |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 1932-6203 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/56768/2/journal.pone.0045489.PDF | |
dc.type.version | PublishedVersion | |