dc.date.accessioned | 2017-01-11T10:14:38Z | |
dc.date.available | 2017-01-11T10:14:38Z | |
dc.date.created | 2016-08-24T09:32:03Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Brandtzaeg, Ole Kristian Johnsen, Elin Follaug Røberg-Larsen, Hanne Seip, Knut Fredrik MacLean, Evan L. Gesquiere, Laurence R. Leknes, Siri Lundanes, Elsa Wilson, Steven Ray Haakon . Proteomics tools reveal startlingly high amounts of oxytocin in plasma and serum. Scientific Reports. 2016, 6:31693 | |
dc.identifier.uri | http://hdl.handle.net/10852/53510 | |
dc.description.abstract | The neuropeptide oxytocin (OT) is associated with a plethora of social behaviors, and is a key topic at the intersection of psychology and biology. However, tools for measuring OT are still not fully developed. We describe a robust nano liquid chromatography-mass spectrometry (nanoLC-MS) platform for measuring the total amount of OT in human plasma/serum. OT binds strongly to plasma proteins, but a reduction/alkylation (R/A) procedure breaks this bond, enabling ample detection of total OT. The method (R/A + robust nanoLC-MS) was used to determine total OT plasma/serum levels to startlingly high concentrations (high pg/mL-ng/mL). Similar results were obtained when combining R/A and ELISA. Compared to measuring free OT, measuring total OT can have advantages in e.g. biomarker studies. | en_US |
dc.language | EN | |
dc.language.iso | en | en_US |
dc.relation.ispartof | Johnsen, Elin Follaug (2017) High performance analytical tools for small molecules in biosamples. Doctoral thesis. http://urn.nb.no/URN:NBN:no-56872 | |
dc.relation.uri | http://urn.nb.no/URN:NBN:no-56872 | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Proteomics tools reveal startlingly high amounts of oxytocin in plasma and serum | en_US |
dc.type | Journal article | en_US |
dc.creator.author | Brandtzaeg, Ole Kristian | |
dc.creator.author | Johnsen, Elin Follaug | |
dc.creator.author | Røberg-Larsen, Hanne | |
dc.creator.author | Seip, Knut Fredrik | |
dc.creator.author | MacLean, Evan L. | |
dc.creator.author | Gesquiere, Laurence R. | |
dc.creator.author | Leknes, Siri | |
dc.creator.author | Lundanes, Elsa | |
dc.creator.author | Wilson, Steven Ray Haakon | |
cristin.unitcode | 185,15,12,63 | |
cristin.unitname | Seksjon for kjemisk livsvitenskap - biomolekyler, bio-inspirerte materialer og bioanalytisk kjemi | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 1375037 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Scientific Reports&rft.volume=6:31693&rft.spage=&rft.date=2016 | |
dc.identifier.jtitle | Scientific Reports | |
dc.identifier.volume | 6 | |
dc.identifier.pagecount | 7 | |
dc.identifier.doi | http://dx.doi.org/10.1038/srep31693 | |
dc.identifier.urn | URN:NBN:no-56702 | |
dc.type.document | Tidsskriftartikkel | en_US |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 2045-2322 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/53510/1/srep31693-brandtzaeg.pdf | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/53510/4/srep31693-s1.pdf | |
dc.type.version | PublishedVersion | |
cristin.articleid | 31693 | |