dc.date.accessioned | 2016-02-11T15:45:56Z | |
dc.date.available | 2016-02-11T15:45:56Z | |
dc.date.created | 2015-03-24T10:40:20Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | Bakke, Siril Skaret Feng, Yuan Zeng Nikolic, Natasa Kase, Eili Tranheim Moro, Cedric Stensrud, Camilla Damlien, Lisbeth Ludahl, Marianne Odnakk Sandbu, Rune Solheim, Brita Marie Rustan, Arild Hjelmesæth, Jøran Thoresen, G. Hege Aas, Vigdis . Myotubes from Severely Obese Type 2 Diabetic Subjects Accumulate Less Lipids and Show Higher Lipolytic Rate than Myotubes from Severely Obese Non-Diabetic Subjects. PLoS ONE. 2015, 10(3) | |
dc.identifier.uri | http://hdl.handle.net/10852/49129 | |
dc.description.abstract | About 80% of patients with type 2 diabetes are classified as overweight. However, only about 1/3 of severely obese subjects have type 2 diabetes. This indicates that several severely obese individuals may possess certain characteristics that protect them against type 2 diabetes. We therefore hypothesized that this apparent paradox could be related to fundamental differences in skeletal muscle lipid handling. Energy metabolism and metabolic flexibility were examined in human myotubes derived from severely obese subjects without (BMI 44±7 kg/m2) and with type 2 diabetes (BMI 43±6 kg/m2). Lower insulin sensitivity was observed in myotubes from severely obese subjects with type 2 diabetes. Lipolysis rate was higher, and oleic acid accumulation, triacylglycerol content, and fatty acid adaptability were lower in myotubes from severely obese subjects with type 2 diabetes compared to severely obese non-diabetic subjects. There were no differences in lipid distribution and mRNA and protein expression of the lipases HSL and ATGL, the lipase cofactor CGI-58, or the lipid droplet proteins PLIN2 and PLIN3. Glucose and oleic acid oxidation were also similar in cells from the two groups. In conclusion, myotubes established from severely obese donors with established type 2 diabetes had lower ability for lipid accumulation and higher lipolysis rate than myotubes from severely obese donors without diabetes. This indicates that a difference in intramyocellular lipid turnover might be fundamental in evolving type 2 diabetes. | en_US |
dc.language | EN | |
dc.language.iso | en | en_US |
dc.publisher | Public Library of Science (PLoS) | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Myotubes from Severely Obese Type 2 Diabetic Subjects Accumulate Less Lipids and Show Higher Lipolytic Rate than Myotubes from Severely Obese Non-Diabetic Subjects | en_US |
dc.type | Journal article | en_US |
dc.creator.author | Bakke, Siril Skaret | |
dc.creator.author | Feng, Yuan Zeng | |
dc.creator.author | Nikolic, Natasa | |
dc.creator.author | Kase, Eili Tranheim | |
dc.creator.author | Moro, Cedric | |
dc.creator.author | Stensrud, Camilla | |
dc.creator.author | Damlien, Lisbeth | |
dc.creator.author | Ludahl, Marianne Odnakk | |
dc.creator.author | Sandbu, Rune | |
dc.creator.author | Solheim, Brita Marie | |
dc.creator.author | Rustan, Arild | |
dc.creator.author | Hjelmesæth, Jøran | |
dc.creator.author | Thoresen, G. Hege | |
dc.creator.author | Aas, Vigdis | |
cristin.unitcode | 185,15,23,30 | |
cristin.unitname | Farmasøytisk biovitenskap | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 1234066 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=PLoS ONE&rft.volume=10&rft.spage=&rft.date=2015 | |
dc.identifier.jtitle | PLoS ONE | |
dc.identifier.volume | 10 | |
dc.identifier.issue | 3 | |
dc.identifier.doi | http://dx.doi.org/10.1371/journal.pone.0119556 | |
dc.identifier.urn | URN:NBN:no-52920 | |
dc.type.document | Tidsskriftartikkel | en_US |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 1932-6203 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/49129/1/EO-PLoSONE%2B2015.pdf | |
dc.type.version | PublishedVersion | |
cristin.articleid | e0119556 | |