dc.date.accessioned | 2016-02-03T13:58:21Z | |
dc.date.available | 2016-03-10T23:31:09Z | |
dc.date.issued | 2015 | |
dc.identifier.uri | http://hdl.handle.net/10852/48904 | |
dc.description.abstract | Dynamic interactions of nuclear lamins with chromatin through lamin-associated domains (LADs) contribute to spatial arrangement of the genome. Here, we provide evidence for prepatterning of differentiation-driven formation of lamin A/C LADs by domains of histone H2B modified on serine 112 by the nutrient sensor O-linked N-acetylglucosamine (H2BS112GlcNAc), which we term GADs. We demonstrate a two-step process of lamin A/C LAD formation during in vitro adipogenesis, involving spreading of lamin A/C–chromatin interactions in the transition from progenitor cell proliferation to cell-cycle arrest, and genome-scale redistribution of these interactions through a process of LAD exchange within hours of adipogenic induction. Lamin A/C LADs are found both in active and repressive chromatin contexts that can be influenced by cell differentiation status. De novo formation of adipogenic lamin A/C LADs occurs nonrandomly on GADs, which consist of megabase-size intergenic and repressive chromatin domains. Accordingly, whereas predifferentiation lamin A/C LADs are gene-rich, post-differentiation LADs harbor repressive features reminiscent of lamin B1 LADs. Release of lamin A/C from genes directly involved in glycolysis concurs with their transcriptional up-regulation after adipogenic induction, and with downstream elevations in H2BS112GlcNAc levels and O-GlcNAc cycling. Our results unveil an epigenetic prepatterning of adipogenic LADs by GADs, suggesting a coupling of developmentally regulated lamin A/C-genome interactions to a metabolically sensitive chromatin modification. | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | Rønningen, Torunn (2016) Metabolic influence on chromatin organization in adipose stem cells. Doctoral thesis. http://urn.nb.no/URN:NBN:no-52728 | |
dc.relation.uri | http://urn.nb.no/URN:NBN:no-52728 | |
dc.rights | Attribution-NonCommercial 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ | |
dc.title | Prepatterning of differentiation-driven nuclear lamin A/C-associated chromatin domains by GlcNAcylated histone H2B | en_US |
dc.type | Journal article | en_US |
dc.creator.author | Rønningen, Torunn | |
dc.creator.author | Shah, Akshay | |
dc.creator.author | Oldenburg, Anja | |
dc.creator.author | Vekterud, Kristin | |
dc.creator.author | Delbarre, Erwan | |
dc.creator.author | Moskaug, Jan Øivind | |
dc.creator.author | Collas, Philippe | |
dc.identifier.cristin | 1295407 | |
dc.identifier.jtitle | Genome Research | |
dc.identifier.volume | 25 | |
dc.identifier.startpage | 1825 | |
dc.identifier.endpage | 1835 | |
dc.identifier.doi | http://dx.doi.org/10.1101/gr.193748.115 | |
dc.identifier.urn | URN:NBN:no-52729 | |
dc.type.document | Tidsskriftartikkel | en_US |
dc.type.peerreviewed | Peer reviewed | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/48904/1/Genome-Res-2015-Ronningen-1825-35.pdf | |
dc.type.version | PublishedVersion | |