dc.date.accessioned | 2015-11-25T15:02:09Z | |
dc.date.available | 2015-11-25T15:02:09Z | |
dc.date.created | 2015-08-25T12:22:37Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | Kong, Xiang Yi Kase, Eili Tranheim Herskedal, Anette Schjalm, Camilla Damme, Markus Nesset, Cecilie Kåsi Thoresen, G. Hege Rustan, Arild Eskild, Winnie . Lack of the lysosomal membrane protein, GLMP, in mice results in metabolic dysregulation in liver. PLoS ONE. 2015, 10(6) | |
dc.identifier.uri | http://hdl.handle.net/10852/47884 | |
dc.description.abstract | Ablation of glycosylated lysosomal membrane protein (GLMP, formerly known as NCU-G1) has been shown to cause chronic liver injury which progresses into liver fibrosis in mice. Both lysosomal dysfunction and chronic liver injury can cause metabolic dysregulation. Glmpgt/gt mice (formerly known as Ncu-g1gt/gtmice) were studied between 3 weeks and 9 months of age. Body weight gain and feed efficiency of Glmpgt/gt mice were comparable to wild type siblings, only at the age of 9 months the Glmpgt/gt siblings had significantly reduced body weight. Reduced size of epididymal fat pads was accompanied by hepatosplenomegaly in Glmpgt/gt mice. Blood analysis revealed reduced levels of blood glucose, circulating triacylglycerol and non-esterified fatty acids in Glmpgt/gt mice. Increased flux of glucose, increased de novo lipogenesis and lipid accumulation were detected in Glmpgt/gt primary hepatocytes, as well as elevated triacylglycerol levels in Glmpgt/gt liver homogenates, compared to hepatocytes and liver from wild type mice. Gene expression analysis showed an increased expression of genes involved in fatty acid uptake and lipogenesis in Glmpgt/gt liver compared to wild type. Our findings are in agreement with the metabolic alterations observed in other mouse models lacking lysosomal proteins, and with alterations characteristic for advanced chronic liver injury. | en_US |
dc.language | EN | |
dc.language.iso | en | en_US |
dc.publisher | Public Library of Science (PLoS) | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.title | Lack of the lysosomal membrane protein, GLMP, in mice results in metabolic dysregulation in liver | en_US |
dc.type | Journal article | en_US |
dc.creator.author | Kong, Xiang Yi | |
dc.creator.author | Kase, Eili Tranheim | |
dc.creator.author | Herskedal, Anette | |
dc.creator.author | Schjalm, Camilla | |
dc.creator.author | Damme, Markus | |
dc.creator.author | Nesset, Cecilie Kåsi | |
dc.creator.author | Thoresen, G. Hege | |
dc.creator.author | Rustan, Arild | |
dc.creator.author | Eskild, Winnie | |
cristin.unitcode | 185,15,29,0 | |
cristin.unitname | Institutt for biovitenskap | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 1259845 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=PLoS ONE&rft.volume=10&rft.spage=&rft.date=2015 | |
dc.identifier.jtitle | PLoS ONE | |
dc.identifier.volume | 10 | |
dc.identifier.issue | 6 | |
dc.identifier.doi | http://dx.doi.org/10.1371/journal.pone.0129402 | |
dc.identifier.urn | URN:NBN:no-51897 | |
dc.type.document | Tidsskriftartikkel | en_US |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 1932-6203 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/47884/2/journal.pone.0129402.pdf | |
dc.type.version | PublishedVersion | |
cristin.articleid | e0129402 | |