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dc.date.accessioned2015-08-28T12:20:12Z
dc.date.available2015-08-28T12:20:12Z
dc.date.created2015-05-29T11:10:06Z
dc.date.issued2015
dc.identifier.citationIversen, Hildegunn Lindbäck, Toril L'Abee-Lund, Trine Marie Roos, Norbert Aspholm, Marina Arnesen, Lotte Pia Stenfors . The gut bacterium Bacteroides thetaiotaomicron influences the virulence potential of the enterohemorrhagic Escherichia coli0103:H25. PLoS ONE. 2015, 10(2), 1-23
dc.identifier.urihttp://hdl.handle.net/10852/45248
dc.description.abstractEnterohemorrhagic E. coli (EHEC) is associated with severe gastrointestinal disease. Upon entering the gastrointestinal tract, EHEC is exposed to a fluctuating environment and a myriad of other bacterial species. To establish an infection, EHEC strains have to modulate their gene expression according to the GI tract environment. In order to explore the interspecies interactions between EHEC and an human intestinal commensal, the global gene expression profile was determined of EHEC O103:H25 (EHEC NIPH-11060424) co-cultured with B. thetaiotaomicron (CCUG 10774) or grown in the presence of spent medium from B. thetaiotaomicron. Microarray analysis revealed that approximately 1% of the EHEC NIPH-11060424 genes were significantly up-regulated both in co-culture (30 genes) and in the presence of spent medium (44 genes), and that the affected genes differed between the two conditions. In co-culture, genes encoding structural components of the type three secretion system were among the most affected genes with an almost 4-fold up-regulation, while the most affected genes in spent medium were involved in chemotaxis and were more than 3-fold up-regulated. The operons for type three secretion system (TTSS) are located on the Locus of enterocyte effacement (LEE) pathogenicity island, and qPCR showed that genes of all five operons (LEE1-LEE5) were up-regulated. Moreover, an increased adherence to HeLa cells was observed in EHEC NIPH-11060424 exposed to B. thetaiotaomicron. Expression of stx2 genes, encoding the main virulence factor of EHEC, was down-regulated in both conditions (co-culture/spent medium). These results show that expression of EHEC genes involved in colonization and virulence is modulated in response to direct interspecies contact between cells, or to diffusible factors released from B. thetaiotaomicron. Such interspecies interactions could allow the pathogen to recognize its predilection site and modulate its behaviour accordingly, thus increasing the efficiency of colonization of the colon mucosa, facilitating its persistence and increasing its virulence potential.en_US
dc.languageEN
dc.language.isoenen_US
dc.publisherPublic Library of Science (PLoS)
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleThe gut bacterium Bacteroides thetaiotaomicron influences the virulence potential of the enterohemorrhagic Escherichia coli0103:H25en_US
dc.typeJournal articleen_US
dc.creator.authorIversen, Hildegunn
dc.creator.authorLindbäck, Toril
dc.creator.authorL'Abee-Lund, Trine Marie
dc.creator.authorRoos, Norbert
dc.creator.authorAspholm, Marina
dc.creator.authorArnesen, Lotte Pia Stenfors
cristin.unitcode185,15,29,30
cristin.unitnameSeksjon for fysiologi og cellebiologi
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.cristin1245110
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=PLoS ONE&rft.volume=10&rft.spage=1&rft.date=2015
dc.identifier.jtitlePLoS ONE
dc.identifier.volume10
dc.identifier.issue2
dc.identifier.doi10.1371/journal.pone.0118140
dc.identifier.urnURN:NBN:no-49494
dc.type.documentTidsskriftartikkelen_US
dc.type.peerreviewedPeer reviewed
dc.source.issn1932-6203
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/45248/2/journal.pone.0118140.pdf
dc.type.versionPublishedVersion
cristin.articleide0118140


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