Abstract
In this study, the parental V79 Chinese hamster lung cell line was compared to the transfected clone V79 (CYP) 2E1 (SULT) 1A1, which expresses the human cytochrome p450 enzyme (CYP) 2E1 and (SULT) 1A1. The transfected cell line was used as a sensitive test model for possible bioactivation of DMF and FFA. Although human sulfotransferase (SULT) often provides a detoxifying pathway for xenobiotics, the enzyme may have a detrimental effect on the cell when certain substituted furans are metabolized. Previous studies using conventional mutagenicity tests were prone to yield false negative results because the test model did not incorporate human CYP and SULT and some substituted furans require human SULT to bioactivate.